Decursin from Angelica gigas Nakai Inhibits B16F10 Melanoma Growth Through Induction of Apoptosis

Journal of Medicinal Food
Byung Soo KimJu-Ock Nam

Abstract

Decursin, a bioactive phytochemical isolated from Angelica gigas Nakai (danggwi), has shown preclinical anticancer efficacy in various cancer models. However, the antitumor effect of decursin in melanoma models remains undefined. The antitumor activities of decursin were investigated in B16F10 cells in vitro and in vivo. In this study, we show that treatment with decursin inhibited cell proliferation in a dose-dependent manner in B16F10 cells, but not in normal cells. Decursin also induced apoptosis in B16F10 cells, as determined by annexin V-staining assay and transferase-mediated nick-end labeling (TUNEL) staining assay. Decursin increased the phosphorylation of p38 as well as the expression of Bax while decreasing the phosphorylation of extracellular signaling-regulated kinase (ERK) and the expression of Bcl-2 in B16F10 cells. Moreover, decursin activated caspase-3 in B16F10 cells and xenograft tumor tissue. Together, these findings support further investigations into the potential use of decursin in the treatment of melanoma cells.

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Citations

Apr 22, 2017·Clinical and Experimental Pharmacology & Physiology·Yue ZhangXiuping Han
Dec 8, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chinreddy Subramanyam ReddySung Cheol Koo
Jun 2, 2017·The Open Virology Journal·Tiantian ZhangKarim Essani
Apr 23, 2016·Oncotarget·Hyeong Sim ChoiSeong-Gyu Ko
May 1, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Bitna KweonSeung-Heon Hong
Sep 26, 2017·Molecular Medicine Reports·Kang Min LeeSeong-Gyu Ko
Aug 14, 2020·ACS Synthetic Biology·Xu-Liang BuMin-Juan Xu

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Methods Mentioned

BETA
ELISA
Assay
flow cytometry

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