Decursin negatively regulates LPS-induced upregulation of the TLR4 and JNK signaling stimulated by the expression of PRP4 in vitro.

Animal Cells and Systems
Muhammad Bilal AhmedYoung Sup Lee

Abstract

The current investigation was carried out to analyze the correlation of bacterial lipopolysaccharide (LPS) and pre-mRNA processing factor 4B (PRP4) in inducing inflammatory response and cell actin cytoskeleton rearrangement in macrophages (Raw 264.7) and colorectal (HCT116) as well as skin cancer (B16-F10) cells. Cell lines were stimulated with LPS, and the expression of PRP4 as well as pro-inflammatory cytokines and proteins like IL-6, IL-1β, TLR4, and NF-κB were assayed. The results demonstrated that LPS markedly increased the expression of PRP4, IL-6, IL-1β, TLR4, and NF-κB in the cells. LPS and PRP4 concomitantly altered the morphology of cells from an aggregated, flattened shape to a round shape. Decursin, a pyranocoumarin from Angelica gigas, inhibited the LPS and PRP4-induced inflammatory response, and reversed the induction of morphological changes. Finally, we established a possible link of LPS with TLR4 and JNK signaling, through which it activated PRP4. Our study provides molecular insights for LPS and PRP4-related pathogenesis and a basis for developing new strategies against metastasis in colorectal cancer and skin melanoma. Our study emphasizes that decursin may be an effective treatment strategy for various cance...Continue Reading

References

Aug 6, 1999·Immunity·T KawaiS Akira
Jun 8, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·W ZhuJ Han
Jun 22, 2001·The Journal of Biological Chemistry·T KojimaM Hagiwara
Nov 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·B L BennettD W Anderson
Sep 7, 2002·International Journal of Cancer. Journal International Du Cancer·Judith H HarmeyZhongyun Dong
Apr 20, 2004·Cancer Cell·Darren M Brown, Erkki Ruoslahti
Sep 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Davide FerrariFrancesco Di Virgilio
Jan 12, 2005·Arthritis Research & Therapy·Taro Kawai, Shizuo Akira
Mar 9, 2005·Cancer Research·Hyung Woo YimYung-Jue Bang
Feb 27, 2008·European Journal of Pharmacology·Tomohiro KawamotoHiroyuki Kimura
Feb 29, 2008·Cytokine·Yong-Chen LuPamela S Ohashi
Mar 26, 2009·International Journal of Colorectal Disease·Peter GassmannJoerg Haier
Oct 11, 2011·Bioscience Reports·Gautam SethiVinay Tergaonkar
May 22, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Libo YanYan Xu
Nov 15, 2017·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Adeeb ShehzadYoung Sup Lee
Nov 22, 2017·Signal Transduction and Targeted Therapy·Ting LiuShao-Cong Sun
Aug 1, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Salman Ul IslamYoung Sup Lee
Oct 23, 2018·European Journal of Microbiology & Immunology·Trudy M Wassenaar, Kurt Zimmermann

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Citations

Aug 23, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tae-Kyeong LeeMoo-Ho Won
Aug 20, 2021·Animal Cells and Systems·Banzragchgarav UnenkhuuHong Seok Kim

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Methods Mentioned

BETA
protein
electrophoresis
transfection
Protein Assay
PCR
confocal microscopy

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