Deep and clear optical imaging of thick inhomogeneous samples.

PloS One
Raphael JorandCorinne Lorenzo

Abstract

Inhomogeneity in thick biological specimens results in poor imaging by light microscopy, which deteriorates as the focal plane moves deeper into the specimen. Here, we have combined selective plane illumination microscopy (SPIM) with wavefront sensor adaptive optics (wao). Our waoSPIM is based on a direct wavefront measure using a Hartmann-Shack wavefront sensor and fluorescent beads as point source emitters. We demonstrate the use of this waoSPIM method to correct distortions in three-dimensional biological imaging and to improve the quality of images from deep within thick inhomogeneous samples.

References

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Citations

Aug 15, 2014·The Analyst·John LimSohail Ahmed
Jan 5, 2014·Protoplasma·Markus Langhans, Tobias Meckel
May 23, 2013·Journal of Microscopy·Annaïck DesmaisonValérie Lobjois
Jan 8, 2014·Microscopy Research and Technique·L SilvestriF S Pavone
Aug 21, 2015·IEEE Transactions on Medical Imaging·Wenxing ZhangPierre Weiss
Sep 21, 2013·Biomedical Optics Express·Diwakar Turaga, Timothy E Holy
Nov 19, 2015·Scientific Reports·Aurore MassonCorinne Lorenzo
Apr 1, 2017·Nature Methods·Na Ji
Jul 10, 2019·Methods and Protocols·Diego Di BattistaOmar E Olarte
May 2, 2018·Applied Physics Letters·Eitan Edrei, Giuliano Scarcelli
Mar 23, 2017·Scientific Reports·Jordi AndillaCorinne Lorenzo
Feb 26, 2019·Biomedical Optics Express·Tobias Meinert, Alexander Rohrbach
Jul 13, 2019·Annual Review of Cell and Developmental Biology·Yinan WanPhilipp J Keller
Feb 11, 2018·Current Opinion in Neurobiology·Cristina Rodríguez, Na Ji

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Methods Mentioned

BETA
light microscopy
confocal microscopy
transfection

Software Mentioned

Imaris
SPIM
Fiji
BitPlane

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