DOI: 10.1101/478925Nov 27, 2018Paper

Deep-learning-based label-free segmentation of cell nuclei in time-lapse refractive index tomograms

BioRxiv : the Preprint Server for Biology
Jimin LeeSung-Joon Ye


In order to identify cell nuclei, fluorescent proteins or staining agents has been widely used. However, use of exogenous agents inevitably prevents from long-term imaging of live cells and rapid analysis, and even interferes with intrinsic physiological conditions. In this work, we proposed a method of label-free segmentation of cell nuclei in optical diffraction tomography images by exploiting a deep learning framework. The proposed method was applied for precise cell nucleus segmentation in two, three, and four-dimensional label-free imaging. A novel architecture with optimised training strategies was validated through cross-modality and cross-laboratory experiments. The proposed method would bring out broad and immediate biomedical applications with our framework publicly available.

Related Concepts

Cell Nucleus
Diagnostic Imaging
Isotope Labeling
Echocardiography, Four-Dimensional
Staining Method
Research Study
Pharmacologic Substance

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