Deep microbial analysis of multiple placentas shows no evidence for a placental microbiome

BJOG : an International Journal of Obstetrics and Gynaecology
A A KupermanO Koren

Abstract

To resolve the controversy regarding the presence of a microbiota in the placenta. Classical and molecular microbiological study. All samples were collected during caesarean section. A total of 28 human placentas and six murine placentas. All 28 human placentas were checked for 16S rRNA gene amplification products. Three locations from four selected human placentas and three 'environmental controls' for each placenta were placed in seven culture media. The four selected human placentas were further analysed using Gram stain, immunohistochemistry for bacteria, electron microscopy, and TaqMan RT-qPCR. Six placentas from three SPF mice were cut into four pieces each, and further analysed for 16S rRNA gene amplification. Microbiological and molecular evidence of bacteria. None of the placental cultures used for the full analysis, or their environmental cultures, was positive for bacterial growth. None of the other methods showed any evidence of bacteria. Immunohistochemistry showed negligible bacterial counts. None of the murine placentas showed evidence of 16S rRNA gene amplification. Our results support that the fetal environment in the womb is sterile. Based on the immunohistochemistry and the limit of detection of the other met...Continue Reading

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Citations

Apr 8, 2020·Proceedings. Biological Sciences·U M Marcinkowska, I J Holzleitner
May 21, 2020·Cell Research·Danping ZhengEran Elinav
Aug 31, 2020·Microbial Biotechnology·Giulia AlessandriMarco Ventura
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Nov 18, 2021·Critical Reviews in Microbiology·Richard CariñoMasahiro Umezaki

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