Deep Phenotypic Mapping of Bacterial Cytoskeletal Mutants Reveals Physiological Robustness to Cell Size

Current Biology : CB
Handuo ShiKerwyn Casey Huang

Abstract

Size is a universally defining characteristic of all living cells and tissues and is intrinsically linked with cell genotype, growth, and physiology. Many mutations have been identified to alter cell size, but pleiotropic effects have largely hampered our ability to probe how cell size specifically affects fundamental cellular properties, such as DNA content and intracellular localization. To systematically interrogate the impact of cell morphology on bacterial physiology, we used fluorescence-activated cell sorting to enrich a library of hundreds of Escherichia coli mutants in the essential cytoskeletal protein MreB for subtle changes in cell shape, cumulatively spanning ∼5-fold variation in average cell volume. Critically, pleiotropic effects in the mutated library are most likely minimized because only one gene was mutated and because growth rate was unaffected, thereby allowing us to query the general effects of morphology on cellular physiology over a large range of cell sizes with high resolution. We discovered linear scaling of the abundance of DNA and the key division protein FtsZ with cell volume, a strong dependency of sensitivity to specific antibiotics on cell width, and a simple correlation between MreB localizatio...Continue Reading

Citations

Jan 1, 2019·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Keisuke KuritaDaisuke Shiomi
Apr 6, 2019·PLoS Computational Biology·Natalie NgKerwyn Casey Huang
Mar 31, 2018·Nature Communications·Alexandre ColavinKerwyn Casey Huang
May 10, 2020·Nature Communications·Qiang DingXiulai Chen
Mar 18, 2020·Nature Communications·Handuo ShiKerwyn Casey Huang
Dec 13, 2019·Biochemical Society Transactions·Paul Richard Jesena Yulo, Heather Lyn Hendrickson
Aug 29, 2019·ELife·Nikola OjkicShiladitya Banerjee
Jun 6, 2018·Frontiers in Microbiology·Tanneke den Blaauwen
Mar 27, 2018·Current Biology : CB·Nika PendeSilvia Bulgheresi
Mar 23, 2021·The ISME Journal·QinQin YuOskar Hallatschek
Apr 22, 2021·The Biochemical Journal·Sarah C Stainbrook, Joseph M Jez
Feb 18, 2021·Molecular Plant-microbe Interactions : MPMI·Wenlong ZhaoXue-Xian Zhang
Sep 8, 2021·Frontiers in Microbiology·Jiawei SunKerwyn Casey Huang
Jun 30, 2021·Annual Review of Cell and Developmental Biology·Ethan C Garner

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