Deep phenotyping of immune cell populations by optimized and standardized flow cytometry analyses

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
Fabien PitoisetMichelle Rosenzwajg

Abstract

Multicolor flow cytometry is a technology of choice for phenotyping of immune cells, and it can be used routinely for the follow up of patients in clinical trials. But it is challenging to define combinations of conjugated antibodies that efficiently allow the detailed analysis of major immune cell subsets and the identification of rare cell populations. In a collaborative work among the Immunology, Immunopathology, Immunotherapy (I3 ) laboratory, and the laboratory of immunomonitoring in oncology (L.I.O), we developed and validated 12 different 10-color flow cytometry panels that allow the deep immunophenotyping of cells from whole blood for the follow up of autoimmune and cancer patients. Here, we describe these optimized flow cytometry panels, showing that they provide the advanced analysis of T cells (including regulatory T cells), B cells, NK cells, MAIT cells, myeloid cells, monocytes, and dendritic cells. Most of the panels have been dried to improve standardization of the labeling and the entire procedure can be performed on less than 2 ml of whole blood. These deep immunophenotyping flow cytometry panels constitute a powerful tool for the monitoring of immune blood cells and will hopefully lead to the discovery of new ...Continue Reading

References

Nov 13, 2004·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Holden T MaeckerJoe Trotter
Jan 13, 2006·Journal of Immunological Methods·Victor AppaySerge Leyvraz
Sep 1, 2006·PLoS Medicine·Dennis McGonagle, Michael F McDermott
Aug 20, 2010·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Yolanda MahnkeMario Roederer
Oct 21, 2010·Nature Immunology·Holden T MaeckerJung-Hua Yeh
May 31, 2011·Autoimmunity Reviews·Makoto MiyaraZahir Amoura
Feb 22, 2012·Nature Reviews. Immunology·Holden T MaeckerRobert Nussenblatt
Mar 2, 2013·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Yolanda D MahnkeMario Roederer
Mar 19, 2013·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Tess BrodieFederica Sallusto
Jan 13, 2015·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Milena HasanUNKNOWN Milieu Intérieur Consortium
May 1, 2015·Frontiers in Immunology·Guillaume ChurlaudDavid Klatzmann
Sep 2, 2015·The Journal of Clinical Investigation·Yiping Yang
Sep 10, 2015·Critical Reviews in Immunology·Marta Consuegra-FernándezFrancisco Lozano
Dec 31, 2015·Annals of Oncology : Official Journal of the European Society for Medical Oncology·S ChampiatA Marabelle
Oct 25, 2016·Autoimmunity Reviews·Christophe JaminJacques-Olivier Pers
Dec 21, 2016·Cell Research·Atsushi Tanaka, Shimon Sakaguchi
Jan 7, 2017·Nature Medicine·Luca GattinoniChiara Bonini
Feb 6, 2017·Nature Reviews. Rheumatology·Medya M ShikhagaieHergen Spits
Mar 7, 2017·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·D F DraxlerR L Medcalf
Apr 4, 2017·Annals of Oncology : Official Journal of the European Society for Medical Oncology·N ChaputF Carbonnel
Apr 28, 2017·International Journal of Laboratory Hematology·A RajabA Porwit
Jun 1, 2017·American Society of Clinical Oncology Educational Book·Jeffrey S Weber
Jan 10, 2018·Cytometry. Part B, Clinical Cytometry·Fabien PitoisetMichelle Rosenzwajg

❮ Previous
Next ❯

Citations

Dec 15, 2018·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Florian Mair
May 3, 2020·Nature Communications·Clélia CoutzacNathalie Chaput
Aug 9, 2020·The Journal of Experimental Medicine·Carla Noemi CastroSeiamak Bahram
Apr 24, 2020·Journal of Immunology Research·Iole MacchiaFrancesca Urbani
Feb 18, 2020·Future Microbiology·Pénélope BourgoinThibaut Markarian
Apr 24, 2020·Computational and Structural Biotechnology Journal·Paulina RybakowskaConcepción Marañón
Apr 24, 2021·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Claudia PasqualiniBirgit Geoerger
May 21, 2021·Frontiers in Immunology·Ulrich BlacheUlrike Koehl
Jun 10, 2021·Reproductive Biomedicine Online·Renata Voltolini VelhoSylvia Mechsner
Nov 30, 2021·Expert Review of Clinical Immunology·Emiko DesvauxPhilippe Moingeon

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.

B-Cell Leukemia (Keystone)

B-cell leukemia includes various types of lymphoid leukemia that affect B cells. Here is the latest research on B-cell leukemia.

Cancer Vaccines

Cancer vaccines are vaccines that either treat existing cancer or prevent development of a cancer.

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.

Related Papers

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
D F DraxlerR L Medcalf
Cytometry. Part a : the Journal of the International Society for Analytical Cytology
Patrick AutissierKenneth C Williams
Clinical Immunology : the Official Journal of the Clinical Immunology Society
Milena HasanMilieu Intérieur Consortium
© 2022 Meta ULC. All rights reserved