Deep Proteomic Deconvolution of Interferons and HBV Transfection Effects on a Hepatoblastoma Cell Line

ACS Omega
Kenneth HodgeTrairak Pisitkun

Abstract

Interferons are commonly utilized in the treatment of chronic hepatitis B virus (HBV) infection but are not effective for all patients. A deep understanding of the limitations of interferon treatment requires delineation of its activity at multiple "omic" levels. While myriad studies have characterized the transcriptomic effects of interferon treatment, surprisingly, few have examined interferon-induced effects at the proteomic level. To remedy this paucity, we stimulated HepG2 cells with both IFN-α and IFN-λ and performed proteomic analysis versus unstimulated cells. Alongside, we examined the effects of HBV transfection in the same cell line, reasoning that parallel IFN and HBV analysis might allow determination of cases where HBV transfection counters the effects of interferons. More than 6000 proteins were identified, with multiple replicates allowing for differential expression analysis at high confidence. Drawing on a compendium of transcriptomic data, as well as proteomic half-life data, we suggest means by which transcriptomic results diverge from our proteomic results. We also invoke a recent multiomic study of HBV-related hepatocarcinoma (HCC), showing that despite HBV's role in initiating HCC, the regulated proteomic...Continue Reading

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Citations

Feb 11, 2021·Biomedicines·Nurbubu T MoldogazievaAlexander A Terentiev

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Datasets Mentioned

BETA
PXD017251

Methods Mentioned

BETA
PCA
acetylation
transfection.
transfection
Assay
protein assay

Software Mentioned

GEM
tidyverse
Piano package
Proteome Discoverer
R
R package “ heatmap . 2
GSEA
DAVID
HT
SEQUEST

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