Deep-red emissive crescent-shaped fluorescent dyes: substituent effect on live cell imaging

ACS Applied Materials & Interfaces
Weimin LiuPengfei Wang

Abstract

A series of crescent-shaped fluorescent dyes (CP1-CP6) were synthesized by hybridizing coumarin and pyronin moieties with different amino substituents at both ends. The molecular structures and photophysical properties of these fluorescent dyes were investigated through X-ray diffraction, absorption spectroscopy, and fluorescence spectroscopy. Results show that the fluorescent dyes exhibited crescent-shaped structures, deep-red emissions (approximately 650 nm), and significant Stokes shifts. In live-cell-imaging experiments, CP1 stains mitochondria, whereas CP3 and CP6 stain the lysosomes in a cytoplasm and the RNA in nucleoli. The relationships between different amino substituent groups and the imaging properties of CP dyes were discussed as well. Additionally, findings from the cytotoxicity and photostability experiments on living cells indicated the favorable biocompatibility and high photostability of the CP dyes.

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Citations

Apr 10, 2016·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Qi LiPengfei Wang
Apr 8, 2016·ACS Applied Materials & Interfaces·Zhiming WangBen Zhong Tang
Aug 4, 2016·The Journal of Organic Chemistry·Guangle NiuPengfei Wang
Sep 24, 2016·Analytical Chemistry·Yonghe TangWeiying Lin
Mar 9, 2017·The Journal of Organic Chemistry·Panpan ZhangPengfei Wang
Sep 6, 2019·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Irina A OkkelmanRuslan I Dmitriev
Apr 18, 2020·Journal of Photochemistry and Photobiology. B, Biology·Murugan SethupathiChellappan Selvaraju
Aug 27, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Fang-Fang GuoZhi-Hong Xu

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