Apr 22, 2016

Deep-sequence profiling of miRNAs and their target prediction in Monotropa hypopitys

Plant Molecular Biology
Anna V ShchennikovaKonstantin G Skryabin

Abstract

Myco-heterotroph Monotropa hypopitys is a widely spread perennial herb used to study symbiotic interactions and physiological mechanisms underlying the development of non-photosynthetic plant. Here, we performed, for the first time, transcriptome-wide characterization of M. hypopitys miRNA profile using high throughput Illumina sequencing. As a result of small RNA library sequencing and bioinformatic analysis, we identified 55 members belonging to 40 families of known miRNAs and 17 putative novel miRNAs unique for M. hypopitys. Computational screening revealed 206 potential mRNA targets for known miRNAs and 31 potential mRNA targets for novel miRNAs. The predicted target genes were described in Gene Ontology terms and were found to be involved in a broad range of metabolic and regulatory pathways. The identification of novel M. hypopitys-specific miRNAs, some with few target genes and low abundances, suggests their recent evolutionary origin and participation in highly specialized regulatory mechanisms fundamental for non-photosynthetic biology of M. hypopitys. This global analysis of miRNAs and their potential targets in M. hypopitys provides a framework for further investigation of miRNA role in the evolution and establishmen...Continue Reading

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Mentioned in this Paper

Metabolic Process, Cellular
Biochemical Pathway
Conserved Sequence
Small Nuclear RNA
RNA library
Genes
RNA, Small Temporal
Nucleic Acid Sequencing
Bio-Informatics
Gene Expression Regulation, Plant

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