Deep sequencing analysis of the circadian transcriptome of the jewel wasp Nasonia vitripennis

BioRxiv : the Preprint Server for Biology
Nathaniel J Davies, Eran Tauber

Abstract

The study of the circadian clock has benefited greatly from using Drosophila as a model system. Yet, accumulating evidence suggests that the fly might not be the canonical insect model. Here, we have analysed the circadian transcriptome of the Jewl wasp Nasonia vitripennis by using RNA-seq in both constant darkness and constant light (in contrast to flies, the wasps are rhythmic under continuous with). We identify approximately 6% of the transcriptome as cycling under constant conditions, revealing a bimodal distribution of phases and low cycling amplitude. We examine the functions under circadian control in Nasonia, identifying clock control of functions such as metabolism, light response, and a variety of neural processes, drawing comparisons between Nasonia and Drosophila . We characterise the transcriptional differences underlying phenotypic differences in free-running circadian behaviour in constant darkness and constant light, revealing significant down-regulation of catabolic processes in constant light. We also profile levels of opsins transcription, gaining insight into how Nasonia responds to light, which is a key question in circadian research in the Hymenoptera. Although there was little similarity between cycling g...Continue Reading

Related Concepts

Metabolic Process, Cellular
Study
Research
WAS gene
Opsins
Genes
Sequence Determinations, RNA
Navitripenicin, Nasonia vitripennis
Circadian Clocks
Transcription, Genetic

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