Deep sequencing and ecological characterization of gut microbial communities of diverse bumble bee species

PloS One
Haw Chuan LimSydney A Cameron

Abstract

Gut bacterial communities of bumble bees are correlated with defense against pathogens. Further understanding this host-microbe association is vitally important as bumble bees are currently experiencing global population declines, potentially due in part to emergent diseases. In this study, we used pyrosequencing and community fingerprinting (ARISA) to characterize the gut microbial communities of nine bumble species from across the Bombus phylogeny. Overall, we delimited 74 bacterial taxa (operational taxonomic units or OTUs) belonging to Betaproteobacteria, Gammaproteobacteria, Bacilli, Actinobacteria, Flavobacteria and Alphaproteobacteria. Each bacterial community was taxonomically simple, containing an average of 1.9 common (relative abundance per sample > 5%) bacterial OTUs. The most abundant and prevalent (occurring in 92% of the samples) bacterial OTU, based on 16S rRNA sequences, closely matched that of the previously described Betaproteobacteria species Snodgrassella alvi. Bacteria that were first described in bee-related external environments dominated a number of gut bacterial communities, suggesting that they are not strictly dependent on the internal gut environment. The ARISA data showed a correlation between bact...Continue Reading

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Citations

Jan 9, 2016·International Journal of Systematic and Evolutionary Microbiology·Waldan K Kwong, Nancy A Moran
May 4, 2016·Nature Reviews. Microbiology·Waldan K Kwong, Nancy A Moran
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Jun 25, 2020·Biotechnology Letters·Bui Thi Thuy DuongDong Van Quyen

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Datasets Mentioned

BETA
CP001562

Methods Mentioned

BETA
amplicon sequencing
amplicon
PCR
light microscopy

Software Mentioned

BOHEMICUS
Automated Ribosomal Intergenic Spacer Analysis ( ARISA )
PERMANOVA
Genemapper
UniFrac
mothur
PERMANOVA +
ARISA
CRWAlign
MrBayes

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