Deep-Sequencing Identification of MicroRNA Biomarkers in Serum Exosomes for Early Pig Pregnancy

Frontiers in Genetics
Chen ZhouLinjun Hong

Abstract

Early pregnancy diagnosis in sows can significantly improve the efficiency of pig industry. Exosomes are membrane-covered nanovesicles that can transport microRNAs (miRNAs) and other molecular signals between cells. In other species, serum exosome-derived miRNAs can serve as good biomarkers of diseases and different physiological states, including pregnancy status. We hypothesized that circulating exosome-derived miRNAs might be used to differentiate the pregnancy status as early as several days after insemination in pigs. To test this hypothesis, we randomly assigned pigs for artificial insemination with fertile or dead semen (control group). Serum samples were obtained from pregnant pigs on days 9, 12, and 15 after insemination and from non-pregnant pigs on days 0, 9, 12, and 15 after insemination. Exosomes were isolated for RNA extraction. The exosomal RNA samples from pigs on day 9 of the estrus cycle and pregnancy were used for small-RNA sequencing. A total 321 miRNAs were identified in all samples. Twenty eight differentially abundant miRNAs were identified between the pregnant and control groups. miRNAs with | log2 (fold change)| > 2 from sequencing results were selected for validation by quantitative reverse-transcripti...Continue Reading

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Citations

Jan 5, 2021·Frontiers in Veterinary Science·Silvia MirettiMario Baratta
May 4, 2021·Frontiers in Veterinary Science·Mallikarjun BidarimathChandrakant Tayade

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Datasets Mentioned

BETA
PRJNA577009

Methods Mentioned

BETA
transmission electron microscopy
Protein Assay
electrophoresis
RNA-Seq
Illumina sequencing
Assay

Software Mentioned

QuantStudio 7 Flex
TargetScan
ZetaView
R package
SPSS
KOBAS
DEGseq
Bowtie

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