Deep Sequencing of Complex Proteoglycans: A Novel Strategy for High Coverage and Site-specific Identification of Glycosaminoglycan-linked Peptides.

Molecular & Cellular Proteomics : MCP
Joshua A KleinJoseph Zaia

Abstract

Proteoglycans are distributed in all animal tissues and play critical, multifaceted, physiological roles. Expressed in a spatially and temporally regulated manner, these molecules regulate interactions among growth factors and cell surface receptors and play key roles in basement membranes and other extracellular matrices. Because of the high degree of glycosylation by glycosaminoglycan (GAG), N-glycan and mucin-type O-glycan classes, the peptide sequence coverage of complex proteoglycans is revealed poorly by standard mass spectrometry-based proteomics methods. As a result, there is little information concerning how proteoglycan site specific glycosylation changes during normal and pathological processes. Here, we developed a workflow to improve sequence coverage and identification of glycosylated peptides in proteoglycans. We applied this workflow to the small leucine-rich proteoglycan decorin and three hyalectan proteoglycans: neurocan, brevican, and aggrecan.We characterized glycosylation of these proteoglycans using LC-MS methods easily implemented on instruments widely used in proteomics laboratories. For decorin, we assigned the linker-glycosite and three N-glycosylation sites. For neurocan and brevican, we identified de...Continue Reading

References

Jul 19, 1996·The Journal of Biological Chemistry·C RetzlerU Rauch
Oct 1, 1996·Current Opinion in Structural Biology·J D Esko, L Zhang
Jan 5, 2002·Cellular and Molecular Life Sciences : CMLS·U RauchX H Zhou
Oct 27, 2004·Proceedings of the National Academy of Sciences of the United States of America·Paul G ScottJordi Bella
Nov 1, 2005·The Journal of Biological Chemistry·Neung-Seon SeoDavid J McQuillan
Apr 27, 2007·Nature·Joseph R BishopJeffrey D Esko
Dec 17, 2009·Methods in Molecular Biology·Joshua E Elias, Steven P Gygi
May 13, 2010·Nature Reviews. Molecular Cell Biology·Chunaram Choudhary, Matthias Mann
Aug 23, 2011·Neuropharmacology·Sabina Berretta
Nov 18, 2011·Therapeutic Advances in Cardiovascular Disease·Sandeep SinglaJawahar L Mehta
Dec 30, 2011·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Atsuko YonedaHinke A B Multhaupt
Jan 21, 2012·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Kornélia BaghyIlona Kovalszky
Apr 28, 2012·The International Journal of Biochemistry & Cell Biology·Renato Frischknecht, Constanze I Seidenbecher
Jan 4, 2013·The FEBS Journal·Anna WadeJoanna J Phillips
Apr 23, 2013·Chinese Journal of Cancer·Xiu-Li Bi, Wancai Yang
Sep 17, 2013·Schizophrenia Research·Harry PantazopoulosSabina Berretta
Nov 14, 2013·PloS One·Christian NiedworokJens W Fischer
Nov 28, 2013·Molecular & Cellular Proteomics : MCP·Gerald W Hart, Lauren E Ball
Jul 24, 2014·Journal of Neuro-oncology·Chrissa A DwyerRussell T Matthews
Oct 22, 2014·Proceedings of the National Academy of Sciences of the United States of America·Jianzhong WenJack E Dixon
Dec 9, 2014·Experimental Neurology·Sujeong YangMaria Grazia Spillantini
Feb 11, 2015·Matrix Biology : Journal of the International Society for Matrix Biology·Hannu JärveläinenThomas N Wight
Feb 24, 2015·Matrix Biology : Journal of the International Society for Matrix Biology·Renato V Iozzo, Liliana Schaefer
Nov 3, 2015·Advanced Drug Delivery Reviews·Thomas NeillRenato V Iozzo
Nov 28, 2015·Glycoconjugate Journal·Han HuJoseph Zaia
Dec 24, 2015·BioMed Research International·Tero A H Järvinen, Stuart Prince
Jan 6, 2016·Mass Spectrometry Reviews·Han HuJoseph Zaia

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Citations

Oct 13, 2019·Molecular & Cellular Proteomics : MCP·Deborah Chang, Joseph Zaia
Jul 1, 2020·Molecular & Cellular Proteomics : MCP·Deborah ChangJoseph Zaia
Mar 27, 2019·Analytical and Bioanalytical Chemistry·Kemal Solakyildirim
Feb 28, 2020·Scientific Reports·Andrea PerssonJonas Nilsson
Sep 1, 2019·Molecular & Cellular Proteomics : MCP·Rekha RaghunathanJoseph Zaia
Sep 5, 2020·Molecular & Cellular Proteomics : MCP·William E Hackett, Joseph Zaia
Dec 1, 2019·Molecular & Cellular Proteomics : MCP·Deborah Chang, Joseph Zaia
Nov 1, 2019·Molecular & Cellular Proteomics : MCP·Rekha RaghunathanJoseph Zaia
Jan 17, 2021·Molecular & Cellular Proteomics : MCP·Deborah ChangJoseph Zaia
Mar 20, 2021·Signal Transduction and Targeted Therapy·Kieran JoyceAbhay Pandit
Mar 25, 2021·Current Protocols·Meizhe WangJoseph Zaia
Mar 25, 2021·Molecular & Cellular Proteomics : MCP·Andrea PerssonGöran Larson
Apr 13, 2021·Molecular & Cellular Proteomics : MCP·Maxwell C McCabeKirk C Hansen
Jul 17, 2019·Journal of Proteome Research·Joshua Klein, Joseph Zaia
Dec 24, 2018·Journal of Proteome Research·Lev I LevitskyMikhail V Gorshkov

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