Deep Sequencing of Small RNAs from Neurosurgical Extracellular Vesicles Substantiates miR-486-3p as a Circulating Biomarker that Distinguishes Glioblastoma from Lower-Grade Astrocytoma Patients.

International Journal of Molecular Sciences
Susannah HallalKimberley L Alexander-Kaufman

Abstract

Extracellular vesicles (EVs) play key roles in glioblastoma (GBM; astrocytoma grade IV) biology and are novel sources of biomarkers. EVs released from GBM tumors can cross the blood-brain-barrier into the periphery carrying GBM molecules, including small non-coding RNA (sncRNA). Biomarkers cargoed in circulating EVs have shown great promise for assessing the molecular state of brain tumors in situ. Neurosurgical aspirate fluids captured during tumor resections are a rich source of GBM-EVs isolated directly from tumor microenvironments. Using density gradient ultracentrifugation, EVs were purified from cavitron ultrasonic surgical aspirate (CUSA) washings from GBM (n = 12) and astrocytoma II-III (GII-III, n = 5) surgeries. The sncRNA contents of surgically captured EVs were profiled using the Illumina® NextSeqTM 500 NGS System. Differential expression analysis identified 27 miRNA and 10 piRNA species in GBM relative to GII-III CUSA-EVs. Resolved CUSA-EV sncRNAs could discriminate serum-EV sncRNA profiles from GBM and GII-III patients and healthy controls and 14 miRNAs (including miR-486-3p and miR-106b-3p) and cancer-associated piRNAs (piR_016658, _016659, _020829 and _204090) were also significantly expressed in serum-EVs. Circ...Continue Reading

References

Jun 9, 1999·International Journal of Cancer. Journal International Du Cancer·S WagnerJ C Tonn
Jan 3, 2003·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·H K KimS Kim
Mar 11, 2005·The New England Journal of Medicine·Roger StuppUNKNOWN National Cancer Institute of Canada Clinical Trials Group
Mar 6, 2009·Genome Biology·Ben LangmeadSteven L Salzberg
Oct 29, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jeffrey I ZwickerBruce Furie
Feb 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Eric M SmallEric N Olson
Mar 13, 2010·Clinica Chimica Acta; International Journal of Clinical Chemistry·Ji-guang ZhangGuang-hai Yang
Mar 23, 2010·Proceedings of the National Academy of Sciences of the United States of America·D Michiel PegtelJaap M Middeldorp
Apr 29, 2010·Current Oncology Reports·Mustafa Khasraw, Andrew B Lassman
Aug 17, 2010·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Soumya A M RaoKumaravel Somasundaram
Dec 9, 2010·Brain Research·Guan SunYongping You
May 24, 2011·Nanomedicine : Nanotechnology, Biology, and Medicine·Rebecca A DragovicIan L Sargent
May 28, 2011·Clinica Chimica Acta; International Journal of Clinical Chemistry·Jia ChengQing-Ning Li
Jul 2, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Takayuki KogureTushar Patel
Jul 12, 2011·Oncology Reports·Richard HummelDamian J Hussey
Sep 20, 2011·Journal of Cellular Biochemistry·Sara Siddiqi, Igor Matushansky
Dec 5, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Hiroko Ohgaki, Paul Kleihues
Dec 12, 2012·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·G HuangX Zhang
Apr 17, 2013·Biochemistry. Biokhimii︠a︡·Yong HuangXiang Chao Cheng
May 15, 2013·BMC Genomics·Xiaoyi HuangLiang Wang
Jul 5, 2013·PloS One·Kristina ScheeKjersti Flatmark
Nov 14, 2013·Journal of Extracellular Vesicles·Rossella CrescitelliJan Lötvall
Nov 20, 2013·Cell Communication and Signaling : CCS·Tatyana A ShtamMichael V Filatov
Apr 18, 2014·Journal of Molecular Cell Biology·Jun-Ming LiaoHua Lu
May 3, 2014·Seminars in Cancer Biology·Jasmina S RedzicXandra O Breakefield
Jul 26, 2014·The Journal of Biological Chemistry·Chuan He YangLawrence M Pfeffer
Nov 30, 2014·Experimental Biology and Medicine·Shin-Yi LiuYu-Jen Chen
Feb 18, 2015·Oncotarget·Maria RavoMaurizio Guida
Apr 24, 2015·Journal of Neuro-oncology·Johnny C AkersClark C Chen
May 17, 2015·Journal of Extracellular Vesicles·María Yáñez-MóOlivier De Wever

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Methods Mentioned

BETA
biopsies
biopsy
PCA
transmission electron microscopy

Software Mentioned

FastQC
FunRich
Vesiclepedia
NTA3
Ingenuity
Oasis2
EV
Excel
CUSA
Ingenuity Pathway

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