Deep Surveying of the Transcriptional and Alternative Splicing Signatures for Decidual CD8+ T Cells at the First Trimester of Human Healthy Pregnancy

Frontiers in Immunology
Weihong ZengYi Lin

Abstract

Decidual CD8+ (dCD8) T cells have been proposed to play important roles in immune protection against the invading pathogens and in tolerance toward the growing semi-allogeneic fetus during early pregnancy. However, their phenotypic and functional characteristics remain poorly defined. Here, we performed the first analysis of the transcriptional and alternative splicing (AS) signatures for human first-trimester dCD8 T cells using high-throughput mRNA sequencing. Our data revealed that dCD8 T cells have distinct transcriptional and AS landscapes when compared with their autologous peripheral blood CD8+ (pCD8) T counterparts. Furthermore, human dCD8 T cells were observed to contain CD8-Treg and effector-memory T-cell subsets, and display enhanced functionality in terms of degranulation and cytokine production on a per-cell basis. Additionally, we have identified the novel splice junctions that use a high ratio of the non-canonical splicing motif GC-AG and found that AS is not a major contributor to the gene expression-level changes between paired pCD8 and dCD8 T cells. Together, our findings not only provide a comprehensive framework of the transcriptional and AS landscapes but also reveal the functional feature of human dCD8 T ce...Continue Reading

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Citations

Apr 25, 2020·American Journal of Reproductive Immunology : AJRI·Xixi HuangMeirong Du
May 29, 2021·Frontiers in Immunology·Kunfeng BaiPhilip C N Chiu
Dec 7, 2021·Frontiers in Immunology·Lilja HardardottirMaria Emilia Solano

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Datasets Mentioned

BETA
GSE105064

Methods Mentioned

BETA
density gradient centrifugation
RNA Assay
fluorescence-activated cell sorting
Illumina sequencing
flow cytometry
PMA
FACS

Software Mentioned

DESeq2
count
Graphpad Prism
rMATS
GSEA analysis
GSEA
MSigDB
R
STAR
FlowJo

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