Deep UV dispersion and absorption spectroscopy of biomolecules

Biomedical Optics Express
Soheil SoltaniFrancisco E Robles

Abstract

Owing to the high precision and sensitivity of optical systems, there is an increasing demand for optical methods that quantitatively characterize the physical and chemical properties of biological samples. Information extracted from such quantitative methods, through phase and/or amplitude variations of light, can be crucial in the diagnosis, treatment and study of disease. In this work we apply a recently developed quantitative method, called ultraviolet hyperspectral interferometry (UHI), to characterize the dispersion and absorbing properties of various important biomolecules. Our system consists of (1) a broadband light source that spans from the deep-UV to the visible region of the spectrum, and (2) a Mach-Zehnder interferometer to gain access to complex optical properties. We apply this method to characterize (and tabulate) the dispersive and absorptive properties of hemoglobin, beta nicotinamide adenine dinucleotide (NAD), flavin adenine dinucleotide (FAD), elastin, collagen, cytochrome c, tryptophan and DNA. Our results shed new light on the complex properties of important biomolecules.

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Citations

Sep 19, 2019·Scientific Reports·Saad AbbasiParsin Haji Reza
Jun 21, 2020·Proceedings of the National Academy of Sciences of the United States of America·Ashkan OjaghiFrancisco E Robles
Dec 5, 2019·Biomedical Optics Express·Saad AbbasiParsin Haji Reza
Apr 4, 2021·International Journal of Molecular Sciences·Ruslan M SarimovSergey V Gudkov
Jun 8, 2021·Extreme Mechanics Letters·M A Bashar EmonM Taher A Saif

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