Deer antler does not represent a typical endochondral growth system: immunoidentification of collagen type X but little collagen type II in growing antler tissue

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
G J RucklidgeS P Robins

Abstract

The collagen isotypes present at early (6 week) and late (5 month) stages of growing deer antler were isolated and identified. Pepsin-digested collagens were separated by differential salt fractionation, SDS-PAGE and Western blotting and subsequently identified by immunostaining. Cyanogen bromide digestion of antler tissue was used to establish a collagen type-specific pattern of peptides, and these were also identified by immunoblotting. Collagen type I was found to be the major collagen in both early- and late-stage antler. Collagen type II was present in the young antler in small amounts but was not confined to the soft "cartilaginous" tip of the antler. Collagen type XI was found in the pepsin digest of the young antler, but collagen type IX was not present at either stage of antler growth. Collagen type X was found in the young antler in all fractions studied. Microscopic study showed that the deer antler did not possess a discrete growth plate as found in endochondral bone growth. Unequivocal immunolocalization of the different collagen types in the antler were unsuccessful. These results show that, despite the presence in the antler of many cartilage collagens, growth does not occur through a simple endochondral process.

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Citations

Nov 30, 2006·Molecular Genetics and Genomics : MGG·Andrea MolnárLászló Orosz
Sep 7, 2011·Glycoconjugate Journal·Peraphan PothacharoenPrachya Kongtawelert
Sep 14, 2013·Journal of Pharmaceutical and Biomedical Analysis·Zhigang SuiYukui Zhang
May 18, 1999·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·H VierimaaJ Risteli
Dec 23, 1998·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·T SzuwartG Clemen
Jun 18, 1999·Matrix Biology : Journal of the International Society for Matrix Biology·K J BosS P Robins
Jun 14, 2005·Current Topics in Developmental Biology·Joanna PriceSteve Allen
Dec 18, 2001·Journal of Biomedical Materials Research·A KapanenJ Tuukkanen
Oct 24, 2006·Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine·E SkiöldebrandS Ekman
May 20, 2021·Scientific Reports·Ranran ZhangXiumei Xing
Nov 6, 2004·Proteomics·Hee Jin ParkByoung Chul Park
May 14, 2005·Matrix Biology : Journal of the International Society for Matrix Biology·Eva KorposFerenc Deák

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