Defect in formation of functional matrix vesicles by growth plate chondrocytes in avian tibial dyschondroplasia: evidence of defective tissue vascularization

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
D NieR E Wuthier

Abstract

Avian tibial dyschondroplasia (ATD), a disease characterized by an almost total lack of mineralization in affected areas of growth plate cartilage, may involve defective matrix vesicle (MV) mineralization. To explore the biochemical defect in ATD, both normal and diseased tissue were analyzed for the amount of isolatable MVs, their chemical composition, and their ability to induce mineral formation. We found significantly fewer MVs in ATD tissue, and in contrast to normal MVs, which rapidly mineralized when incubated in synthetic cartilage lymph, those isolated from ATD lesions induced only limited mineralization even after prolonged incubation. Analysis by detergent extraction revealed a nearly dysfunctional nucleational core in ATD MVs. Thus, in ATD tissue, there is a defect in the formation of MVs, and those that form are nearly inactive. There were also alterations in the lipid-dependent Ca2+(-)binding proteins (annexins) in ATD MVs. There were lower levels of annexins II and VI in endogenously produced collagenase-released matrix vesicles (CRMVs), but not in matrix vesicle-enriched microsomes (MVEMs) produced by tissue homogenization. These findings indicate that there is insufficient Ca2+ in ATD cells to enable incorporat...Continue Reading

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Citations

Apr 7, 2018·Acta Histochemica Et Cytochemica·Tomohiro TakenawaJunichiro Iida
Sep 1, 1996·Developmental Dynamics : an Official Publication of the American Association of Anatomists·J WuR M Leach
Oct 23, 1997·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·K OhyamaI M Shapiro
Jun 6, 2000·Biochimica Et Biophysica Acta·D JefferiesC Farquharson

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