Defective expression of Galpha12 in the testes of azoospermia patients and in the spermatozoa with low motility.

Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte
Yanqiu HuJiahao Sha

Abstract

Antibody to the Galpha12-subunit of guanine nucleotide regulatory proteins was used to determine whether the Galpha12 is present in adult human spermatogenic cells and to determine its role in dyszoospermia. Immunoblots from testes and spermatozoa demonstrated the presence of Galpha12 in the samples. Immunohistochemical analyses of testes found that Galpha12 was expressed in the cytoplasm of Leydig cells and was expressed in spermatids from the elongating Sb phase to mature sperm. Indirect immunofluorescence of human spermatozoa revealed the presence of Galpha12 in the neck region and the midpiece of the sperm. Galpha12 in spermatids and spermatozoa partially co-localized with F-actin and alpha-tubulin. Immunohistochemical analyses of tissues from three patients with non-obstructive azoospermia showed abnormal expression of Galpha12 in more than 45% of spermatids. Furthermore, Western blots and indirect immunofluorescence found defective expression of Galpha12 in low-motility spermatozoa with midpieces that were bent on themselves. Therefore, it suggests that Galpha12 plays a role in polarity and tail formation as spermatids mature. Furthermore, Galpha12 may be a candidate protein responsible for azoospermia caused by spermatog...Continue Reading

References

Oct 1, 1992·European Journal of Pharmacology·K P LeschD L Murphy
Jul 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M P Strathmann, M I Simon
Oct 20, 1995·The Journal of Biological Chemistry·A M BuhlG L Johnson
Feb 15, 1994·Biochemical and Biophysical Research Communications·K SpicherG Schultz
Feb 1, 1993·Molecular and Cellular Biology·A M ChanS A Aaronson
Apr 29, 1998·Human Reproduction·R Haimov-Kochman, E Ben-Chetrit
Nov 11, 1998·Developmental Biology·G ManandharG Schatten
Jan 1, 1999·Molecular Human Reproduction·J P FouquetR Melki
Mar 2, 1999·Molecular Human Reproduction·F MerletP de Mazancourt
Jun 11, 1999·The Journal of Biological Chemistry·A GohlaG Schultz
May 19, 2000·The Journal of Biological Chemistry·R VaiskunaiteT A Voyno-Yasenetskaya
Nov 25, 2000·Biology of Reproduction·C LécuyerR Rousseaux-Prévost
Feb 17, 2001·Journal of Cellular Biochemistry·J M DermottN Dhanasekaran
Apr 12, 2002·The International Journal of Biochemistry & Cell Biology·David J ArmstrongStephen Kaye
Apr 27, 2002·The Journal of Biological Chemistry·Thomas E MeigsPatrick J Casey
Sep 5, 2002·Molecular Reproduction and Development·Abraham L Kierszenbaum
Oct 12, 2004·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Arzu VicdanEran Sözen

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Citations

Dec 3, 2014·Current Opinion in Immunology·Jessica F Brinkworth, Luis B Barreiro
Apr 26, 2017·American Journal of Medical Genetics. Part a·Andrea C YuChristine M Armour
Oct 12, 2007·Biology of Reproduction·Sarah E FiedlerDaniel W Carr
Jun 15, 2018·Molecular Medicine Reports·Tiancheng ZhangFudong Yu

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