Nov 14, 2012

Defective FGF signaling causes coloboma formation and disrupts retinal neurogenesis

Cell Research
Shuyi ChenTing Xie

Abstract

The optic fissure (OF) is a transient opening on the ventral side of the developing vertebrate eye that closes before nearly all retinal progenitor cell differentiation has occurred. Failure to close the OF results in coloboma, a congenital disease that is a major cause of childhood blindness. Although human genetic studies and animal models have linked a number of genes to coloboma, the cellular and molecular mechanisms driving the closure of the OF are still largely unclear. In this study, we used Cre-LoxP-mediated conditional removal of fibroblast growth factor (FGF) receptors, Fgfr1 and Fgfr2, from the developing optic cup (OC) to show that FGF signaling regulates the closing of the OF. Our molecular, cellular and transcriptome analyses of Fgfr1 and Fgfr2 double conditional knockout OCs suggest that FGF signaling controls the OF closure through modulation of retinal progenitor cell proliferation, fate specification and morphological changes. Furthermore, Fgfr1 and Fgfr2 double conditional mutant retinal progenitor cells fail to initiate retinal ganglion cell (RGC) genesis. Taken together, our mouse genetic studies reveal that FGF signaling is essential for OF morphogenesis and RGC development.

  • References82
  • Citations9

References

  • References82
  • Citations9

Citations

Mentioned in this Paper

Vertebrates
Fibroblast Growth Factor Receptor 1
FGFR1 wt Allele
FGFR2 gene
Human Genetics
Fibroblast Growth Factor Receptor 2
Congenital Disorders
Blind Vision
Fibroblast Growth Factor Binding
Entire Optic Cup

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