PMID: 9420235Jan 7, 1998Paper

Defective growth correlates with reduced accumulation of a viral DNA replication protein after low-multiplicity infection by a human cytomegalovirus ie1 mutant

Journal of Virology
R F Greaves, E S Mocarski

Abstract

To investigate the importance of the IE1 p72 regulatory protein during human cytomegalovirus replication, a recombinant virus unable to synthesize IE1 p72 was constructed. The Towne strain mutant CR208 lacked exon 4 of the major immediate-early gene and was isolated and complemented in an IE1-expressing immortalized human fibroblast line (ihfie1.3). Replication of CR208 in primary human fibroblasts was completed after an input multiplicity of 10 PFU/cell but was severely-impaired at 0.1 PFU/cell. CR208 formed plaques with lower efficiency on primary fibroblasts than on ihfiel.3 cells, and the relationship between the CR208 inoculum size and the resulting number of undersized plaques was nonlinear, indicating that multiple particles of CR208 were required to initiate lytic replication in a single primary fibroblast. After infection of primary fibroblasts with CR208 at 5 PFU/cell, a normal pattern of viral antigens was detected, although IE1 p72 was absent. During lower-multiplicity infections, IE2 protein was consistently detected at similar levels in a similar proportion of CR208-infected cells relative to the case for a Towne infection, but many fewer CR208-infected cells contained the ppUL44 polymerase accessory protein when ...Continue Reading

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Citations

Dec 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·W A Bresnahan, T E Shenk
Jun 29, 2001·Transplant Infectious Disease : an Official Journal of the Transplantation Society·P ReinkeH D Volk
Dec 24, 2003·International Reviews of Immunology·Jonathan P Castillo, Timothy F Kowalik
Apr 29, 2004·Journal of Virology·Philip E LashmitMark F Stinski
Dec 2, 2004·Proceedings of the National Academy of Sciences of the United States of America·Michael NevelsThomas Shenk
Feb 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·Christina PaulusMichael Nevels
Jul 23, 2008·The Journal of Biological Chemistry·Nicholas J MacheskyJoanne Trgovcich
Mar 18, 2009·Virus Genes·Lars DölkenUlrich H Koszinowski
Aug 8, 2009·Future Microbiology·Manfred Marschall, Thomas Stamminger
Aug 7, 2009·Journal of Virology·Noam Stern-GinossarOfer Mandelboim
Jul 24, 2013·Proceedings of the National Academy of Sciences of the United States of America·Einat ZalckvarMichael Nevels
Jul 28, 1999·FEMS Microbiology Reviews·C Sweet
Feb 7, 2004·The Journal of Biological Chemistry·Sheng-Hao ChaoJeremy S Caldwell

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