Defective immunometabolism pathways in cystic fibrosis macrophages.

Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society
Kaitlin HamiltonAmal O Amer

Abstract

Mitochondria play a key role in immune defense pathways, particularly for macrophages. We and others have previously demonstrated that cystic fibrosis (CF) macrophages exhibit weak autophagy activity and exacerbated inflammatory responses. Previous studies have revealed that mitochondria are defective in CF epithelial cells, but to date, the connection between defective mitochondrial function and CF macrophage immune dysregulation has not been fully elucidated. Here, we present a characterization of mitochondrial dysfunction in CF macrophages. Mitochondrial function in wild-type (WT) and CF F508del/F508del murine macrophages was measured using the Seahorse Extracellular Flux analyzer. Mitochondrial morphology was investigated using transmission electron and confocal microscopy. Mitochondrial membrane potential (MMP) as well as mitochondrial reactive oxygen species (mROS) were measured using TMRM and MitoSOX Red fluorescent dyes, respectively. All assays were performed at baseline and following infection by Burkholderia cenocepacia, a multi-drug resistant bacterium that causes detrimental infections in CF patients. We have identified impaired oxygen consumption in CF macrophages without and with B. cenocepacia infection. We also...Continue Reading

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Citations

Apr 13, 2021·The International Journal of Biochemistry & Cell Biology·Rocío GarcíaÁngel G Valdivieso

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