Defective lipoprotein sorting induces lolA expression through the Rcs stress response phosphorelay system.

Journal of Bacteriology
Kazuyuki TaoHajime Tokuda

Abstract

The Escherichia coli LolA protein is a lipoprotein-specific chaperone that carries lipoproteins from the inner to the outer membrane. A dominant negative LolA mutant, LolA(I93C/F140C), in which both (93)Ile and (140)Phe are replaced by Cys, binds tightly to the lipoprotein-dedicated ABC transporter LolCDE complex on the inner membrane and therefore inhibits the detachment of outer membrane-specific lipoproteins from the inner membrane. We found that the expression of this mutant strongly induced lolA gene transcription. The depletion of the LolA or LolB protein also triggered lolA gene transcription, indicating that the inhibition of outer membrane lipoprotein transport triggers lolA transcription. To elucidate the mechanism, we isolated mutants that are unable to induce lolA transcription using the lacZ gene fused to the lolA promoter as a reporter and found that the Rcs phosphorelay system directly regulates lolA transcription. An outer membrane lipoprotein, RcsF, was essential for this activation, while the coactivator RcsA was dispensable. Taking the observation that an RcsF mutant localized in the inner membrane constitutively activated the Rcs phosphorelay system into consideration, the results shown here strongly suggest...Continue Reading

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Citations

Dec 20, 2014·Cell·Seung-Hyun ChoJean-François Collet
Jun 14, 2018·Annual Review of Microbiology·Erin WallSusan Gottesman
Jun 1, 2019·Nature Reviews. Microbiology·Angela M Mitchell, Thomas J Silhavy
Jul 5, 2017·Journal of Bacteriology·Géraldine Laloux, Jean-François Collet
Sep 30, 2016·Proceedings of the National Academy of Sciences of the United States of America·Joseph M BollM Stephen Trent
Nov 2, 2017·Frontiers in Microbiology·Xiao-Peng Guo, Yi-Cheng Sun
Apr 30, 2020·Journal of Microbiology and Biotechnology·Zhenyue FengYudong Cui
Mar 6, 2021·Current Opinion in Microbiology·Jessica El RayesJean-François Collet
Mar 5, 2021·Frontiers in Microbiology·Jiao MengJingyu Chen
Apr 13, 2021·Frontiers in Molecular Biosciences·William Dowhan, Mikhail Bogdanov
Apr 27, 2021·Current Opinion in Microbiology·Shreya SahaAnna Konovalova
Aug 6, 2021·Proceedings of the National Academy of Sciences of the United States of America·Muralidhar TataAnna Konovalova

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