Defective long-term repopulating ability in hematopoietic stem cells lacking the Polycomb-group gene rae28

European Journal of Haematology
Ji Yoo KimYoshihiro Takihara

Abstract

The rae28 gene (rae28) is a member of a Polycomb-group (PcG) complex 1, which is known to help maintain transcription states once these have been initiated, by generating heritable higher-order chromatin structures. In this study, we examined the capacity of rae28-deficient (rae28-/-) hematopoietic stem cells (HSCs) to generate long-term marrow reconstitution. rae28-/- fetal liver cells containing 20 competitive repopulation units (CRUs) were able to support the survival of lethally irradiated congenic mice for as long as 6 months. The marrow reconstituted with the rae28-/- cells, however, could not increase HSCs efficiently. This was evidenced by its inability to reconstitute marrow in serial transplantation experiments, as well as by the reduction in HSC-enriched Lin- c-kit+ Sca-1high+ subpopulation in the bone marrow cells. Moreover, the reconstituted marrow produced less than half of the peripheral blood cells in each of the lineages examined. We also monitored the mean stem cell activity (MAS). MAS of rae28-/- CRUs was progressively reduced after transplantation, and after 12 months it was reduced to one-tenth of that of the wild-type. These in vivo results clearly indicate that rae28 is indispensable for the long-term rep...Continue Reading

References

Feb 1, 1990·Genes & Development·C T Jordan, I R Lemischka
May 1, 1990·The Journal of Experimental Medicine·G Keller, R Snodgrass
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S J SzilvassyC J Eaves
Apr 1, 1997·Current Opinion in Genetics & Development·V Pirrotta
May 5, 1997·FEBS Letters·M OkabeY Nishimune
Aug 1, 1997·Current Opinion in Genetics & Development·A Gould
Jun 26, 1998·Nature·Y Katoh-FukuiT Higashinakagawa
Oct 31, 1998·Current Opinion in Genetics & Development·M H Sieweke, T Graf
Sep 12, 2000·The American Journal of Pathology·F M RaaphorstC J Meijer
Feb 13, 2001·Current Biology : CB·S J Morrison
May 5, 2001·Immunity·E LagasseI L Weissman
Mar 20, 2002·The Journal of Experimental Medicine·Hideaki OhtaYoshihiro Takihara
Aug 9, 2002·Molecular and Cellular Biology·Stuart S LevineRobert E Kingston
Sep 14, 2002·Science·Miguel Ramalho-SantosDouglas A Melton
Sep 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Min LuYoshimoto Katsura

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Citations

Jan 29, 2008·International Journal of Hematology·Yoshihiro Takihara
Jan 25, 2005·Nature Immunology·Andrew W DuncanTannishtha Reya
Jul 25, 2008·Proceedings of the National Academy of Sciences of the United States of America·Motoaki OhtsuboYoshihiro Takihara
Feb 9, 2005·DNA and Cell Biology·Jesús GilGordon Peters
Jul 22, 2010·Development, Growth & Differentiation·Takaaki KonumaAtsushi Iwama
Jun 21, 2007·PloS One·Pearl A CampbellMichael A Rudnicki
May 26, 2005·International Journal of Hematology·Atsushi IwamaHiromitsu Nakauchia
Apr 28, 2011·International Journal of Hematology·Karin Klauke, Gerald de Haan
Jul 14, 2010·Annual Review of Cell and Developmental Biology·Julie A Lessard, Gerald R Crabtree
Jun 20, 2007·Experimental Hematology·Sonia CellotGuy Sauvageau
May 31, 2007·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Takeshi UedaRikiro Fukunaga
Aug 22, 2006·Current Opinion in Genetics & Development·Omobolaji O Akala, Michael F Clarke
Apr 18, 2016·International Journal of Hematology·Emi Takamatsu-Ichihara, Issay Kitabayashi
Feb 23, 2008·Current Opinion in Cell Biology·Alexandra M Pietersen, Maarten van Lohuizen
Aug 19, 2010·Blood·Daniel Martin-PerezMargarita Sanchez-Beato
Jul 17, 2016·International Journal of Hematology·Shin'ichiro YasunagaYoshihiro Takihara
Jan 15, 2017·Experimental Hematology·Miguel Vidal, Katarzina Starowicz
Oct 21, 2018·The Journal of Cell Biology·Valerio Di CarloLuciano Di Croce
Nov 15, 2007·Cytogenetic and Genome Research·K MiharaA Kimura
Jul 9, 2020·Experimental & Molecular Medicine·Seok-Jin Kang, Taehoon Chun

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