Defective pH regulation of acidic compartments in human breast cancer cells (MCF-7) is normalized in adriamycin-resistant cells (MCF-7adr)

Biochemistry
M SchindlerS M Simon

Abstract

Alkalinization of normally acidic intracellular compartments or acidification of a mildly alkaline cytoplasm by biochemical or genetic manipulation has been demonstrated to inhibit both endocytosis and secretion (Tartakoff, 1983a; Cosson et al., 1989; Mellman et al., 1986; Davoust et al., 1987; Cosson et al., 1989; van Deurs et al., 1989; Maxfield & Yamashiro, 1991; Hansen et al., 1993). These results provide the basis for the conclusion that the maintenance of pH gradients between acidic vesicular compartments and a mildly alkaline cytoplasm is an essential biochemical requirement for the correct functioning of the endocytotic and secretory machinery. Tumor cells have been shown to have an abnormally acidic cytoplasmic pH (Warburg, 1956; Simon & Schindler, 1994). Here we report that the intracellular vesicular compartments in tumor cells (MCF-7) derived from a human breast cancer fail to acidify. This failure results in a significant decrease in the pH gradient (0.9 pH unit) between the vesicular luminal compartments and the cytoplasm. These defects are correlated with a disruption in the organization and function of the trans-Golgi network (TGN) and the pericentriolar recycling compartment (PRC). In marked distinction, drug-r...Continue Reading

References

May 1, 1991·Analytical Biochemistry·J E WhitakerF G Prendergast
Jan 1, 1989·International Review of Cytology·B van DeursK Sandvig
Jan 1, 1986·Annual Review of Biochemistry·I MellmanA Helenius
Sep 15, 1987·Biochemical Pharmacology·W T Beck
Dec 1, 1987·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M C WillinghamI H Pastan
Dec 1, 1994·The Journal of General Physiology·G R EhringM D Cahalan
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·S M Simon, M Schindler
Aug 1, 1994·The American Journal of Physiology·D B LuckieJ J Wine
Dec 15, 1994·International Journal of Cancer. Journal International Du Cancer·A MolinariG Arancia
Oct 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·G A AltenbergL Reuss
Feb 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·S SimonM Schindler
Jan 1, 1994·The American Journal of Physiology·R W Van Dyke, J D Belcher
Feb 24, 1956·Science·O WARBURG

❮ Previous
Next ❯

Citations

Apr 9, 2013·Naunyn-Schmiedeberg's Archives of Pharmacology·Martin WernerMartin Hohenegger
Apr 5, 2013·Analytical and Bioanalytical Chemistry·María J MarínDavid A Russell
Mar 22, 2007·Pharmaceutical Research·Yuping GongJeffrey P Krise
Mar 31, 2000·Pharmacology & Therapeutics·A K LarsenA Skladanowski
Apr 2, 1999·Trends in Pharmacological Sciences·P D Roepe, J A Martiney
Sep 28, 1998·Biochimica Et Biophysica Acta·A K Larsen, A Skladanowski
May 11, 1999·Drug Discovery Today·S M Simon
Feb 14, 2002·Trends in Molecular Medicine·Jonathan M Rhodes, Barry J Campbell
Nov 2, 2012·Bioconjugate Chemistry·Sung Bae KimMasaki Torimura
Aug 5, 2011·Inorganic Chemistry·Jy D ChartresPaul V Bernhardt
Nov 19, 2013·Journal of the American Chemical Society·Hyung Joong KimHwan Myung Kim
Dec 1, 2005·Chemical Communications : Chem Comm·Zongren Zhang, Samuel Achilefu
Sep 14, 2007·Chemical Communications : Chem Comm·Bo TangLiguo An
Oct 30, 2012·Organic & Biomolecular Chemistry·Emmanuel E NekongoVladimir V Popik
Jan 26, 2011·Proceedings of the National Academy of Sciences of the United States of America·Pavitra KannanMatthew D Hall
Jul 27, 2012·Journal of Biomolecular Structure & Dynamics·Cui-Li YuanKe-Zhi Wang
Jun 13, 1998·The Journal of Experimental Medicine·N AltanS M Simon
Nov 18, 2004·Journal of the National Cancer Institute·Francesca LucianiStefano Fais
Apr 20, 2004·Anti-cancer Drugs·Tobias HenningBernhard Wolf
Jul 9, 2005·Cancer Cell International·Valeria Ponce de León, Raúl Barrera-Rodríguez
Nov 26, 2009·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Libin Yuan, Carlos R Morales
Mar 25, 2006·Future Oncology·Angelo De Milito, Stefano Fais
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·N AltanS M Simon
Apr 12, 2014·Chemical Society Reviews·Zhigang YangJong Seung Kim
Feb 8, 2011·Dalton Transactions : an International Journal of Inorganic Chemistry·Xiaolin ZhangChunying Duan
May 24, 2012·Expert Opinion on Drug Metabolism & Toxicology·Randall LoganJeffrey P Krise
Jun 17, 2005·Expert Opinion on Pharmacotherapy·Angelo De Milito, Stefano Fais
Mar 15, 2011·Drug Metabolism Reviews·Fang ZhouMichael S Roberts

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.