Defective secretion of islet hormones in chromogranin-B deficient mice.

PloS One
Stefanie ObermüllerSebastian Barg

Abstract

Granins are major constituents of dense-core secretory granules in neuroendocrine cells, but their function is still a matter of debate. Work in cell lines has suggested that the most abundant and ubiquitously expressed granins, chromogranin A and B (CgA and CgB), are involved in granulogenesis and protein sorting. Here we report the generation and characterization of mice lacking chromogranin B (CgB-ko), which were viable and fertile. Unlike neuroendocrine tissues, pancreatic islets of these animals lacked compensatory changes in other granins and were therefore analyzed in detail. Stimulated secretion of insulin, glucagon and somatostatin was reduced in CgB-ko islets, in parallel with somewhat impaired glucose clearance and reduced insulin release, but normal insulin sensitivity in vivo. CgB-ko islets lacked specifically the rapid initial phase of stimulated secretion, had elevated basal insulin release, and stored and released twice as much proinsulin as wildtype (wt) islets. Stimulated release of glucagon and somatostatin was reduced as well. Surprisingly, biogenesis, morphology and function of insulin granules were normal, and no differences were found with regard to beta-cell stimulus-secretion coupling. We conclude that ...Continue Reading

References

Aug 1, 1992·Neuroscience·H Winkler, R Fischer-Colbrie
Jan 1, 1991·Trends in Biochemical Sciences·W B HuttnerP Rosa
Feb 12, 1993·Science·C Nanavati, J M Fernandez
Apr 30, 1996·Proceedings of the National Academy of Sciences of the United States of America·S Natori, W B Huttner
Mar 10, 2000·Regulatory Peptides·E KarlssonS Sandler
Sep 1, 2000·Seminars in Cell & Developmental Biology·M MolineteP A Halban
Apr 27, 2002·Pflügers Archiv : European journal of physiology·Charlotta S OlofssonLena Eliasson
Jul 24, 2002·Proceedings of the National Academy of Sciences of the United States of America·Xiaorong ZhuDonald F Steiner
Aug 24, 2002·Science·Noriko TakahashiHaruo Kasai
Dec 12, 2002·Trends in Endocrinology and Metabolism : TEM·Robert Day, Sven Ulrik Gorr
Mar 21, 2003·The New England Journal of Medicine·Laurent TaupenotDaniel T O'Connor
Apr 5, 2003·Current Biology : CB·Takashi Tsuboi, Guy A Rutter
Jun 20, 2003·The New England Journal of Medicine·Hans Scherübl, Patricia Grabowski
Aug 7, 2003·The Journal of Biological Chemistry·Yang Hoon HuhSeung Hyun Yoo
Jan 22, 2004·Journal of Cell Science·Maria Luisa MalosioJacopo Meldolesi
Jun 16, 2004·Proceedings of the National Academy of Sciences of the United States of America·Li MaLouis H Philipson
Feb 3, 2005·Biological Reviews of the Cambridge Philosophical Society·Karen B Helle
Jul 12, 2005·The Journal of Clinical Investigation·Nitish R MahapatraSushil K Mahata
Jul 29, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Taeyoon KimY Peng Loh
Sep 6, 2005·Journal of Cell Science·Stefanie ObermüllerSebastian Barg
Mar 1, 2006·Diabetes·Darren J MichaelRobert H Chow
Jun 30, 2006·Current Opinion in Cell Biology·Barbara BorgonovoMichele Solimena
Oct 3, 2006·Cell Metabolism·Patrick E MacDonaldPatrik Rorsman
Oct 3, 2007·Cell Metabolism·Nan GaoKlaus H Kaestner
Mar 28, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Monica S MontesinosRicardo Borges
Aug 30, 2008·Regulatory Peptides·G M Portela-GomesS K Mahata

❮ Previous
Next ❯

Citations

Feb 12, 2014·Proceedings of the National Academy of Sciences of the United States of America·Sinisa HrvatinDouglas A Melton
Nov 26, 2010·Current Hypertension Reports·Kuixing ZhangDaniel T O'Connor
May 6, 2011·Diabetologia·L RussoUNKNOWN ISPED Early Diabetes Study Group
Aug 25, 2011·Endocrine Reviews·Alessandro BartolomucciStephen R J Salton
Mar 15, 2012·Journal of Molecular Neuroscience : MN·Natalia DomínguezRicardo Borges
Dec 23, 2011·Diabetologia·M B HoppaP Rorsman
May 3, 2011·Journal of Neural Transmission·Michael WillisJosef Marksteiner
Sep 19, 2012·Cellular and Molecular Life Sciences : CMLS·Natalie Porat-ShliomRoberto Weigert
Jul 9, 2010·Trends in Endocrinology and Metabolism : TEM·Jakob Suckale, Michele Solimena
May 12, 2010·Journal of Neurochemistry·Ricardo BorgesJosé D Machado
May 1, 2015·Revista Portuguesa De Pneumologia·Vitor SousaLina Carvalho
Jun 19, 2013·PloS One·Jialin GaoHuiwen Zhang
Sep 27, 2014·Behavioural Brain Research·Daniel PeredaRicardo Borges
Sep 20, 2018·Diabetes, Obesity & Metabolism·Yi-Chun ChenC Bruce Verchere
Feb 24, 2016·PloS One·Ahmed M AbdellatifSatoru Takahashi
Nov 4, 2010·Cellular and Molecular Neurobiology·José D MachadoRicardo Borges
Oct 13, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jésica Díaz-VeraRicardo Borges
Feb 6, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Samira FargaliStephen R Salton
Jan 1, 2020·IUBMB Life·Fanny LaguerreMaité Montero-Hadjadje
Feb 23, 2019·Cell Communication and Signaling : CCS·Jaime Santo-DomingoAndreas Wiederkehr
Dec 2, 2014·Biomolecular Concepts·Judith Estevez-HerreraRicardo Borges
Feb 27, 2016·The Journal of Biological Chemistry·Taiyi KuoDomenico Accili
Jun 12, 2019·Journal of Cell Science·Shelby C BearrowsSamuel B Stephens
Nov 21, 2018·Life Science Alliance·Gaya P YadavQiu-Xing Jiang
Jan 5, 2018·Molecular Biology of the Cell·Jia LiJun Xia
Jan 5, 2020·Cell and Tissue Research·Leandro Castañeyra-RuizRicardo Borges
Sep 14, 2016·The Biochemical Journal·Pia V RöderWeiping Han
Nov 28, 2017·Journal of Neurochemistry·Natalia DominguezMatthijs Verhage
Nov 5, 2020·Cellular and Molecular Life Sciences : CMLS·Muhmmad Omar-Hmeadi, Olof Idevall-Hagren
May 6, 2021·Metabolites·Nicholas NorrisMelkam Alamerew Kebede
Jul 31, 2021·World Journal of Diabetes·Zoltan HeroldAniko Somogyi
Aug 27, 2021·Metabolites·Mark GermanosMelkam A Kebede

❮ Previous
Next ❯

Methods Mentioned

BETA
enzyme-linked immunosorbent assay
electron microscopy
ELISA
confocal microscopy

Related Concepts

Related Feeds

Biomarkers for Type 2 Diabetes

Biomarkers can help understand chronic diseases and assist in risk prediction for prevention and early detection of diseases. Here is the latest research on biomarkers in type 2 diabetes, a disease in which the body is unable to produce or properly use insulin.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.