PMID: 6160916Nov 1, 1980Paper

Defective terminal differentiation in culture as a consistent and selectable character of malignant human keratinocytes

Cell
James G Rheinwald, M A Beckett

Abstract

Culture conditions can be manipulated to vary the rate at which keratinocytes (stratified squamous epithelial cells) become committed to differentiate terminally. When deprived of anchorage in semisolid medium, normal human keratinocytes irreversibly lose the ability to reinitiate growth in surface culture with a t1/2 of 3 hr and then proceed to form cornified envelopes. We examined six established lines from human squamous cell carcinomas (SCCs) for defects in this function. One SCC line which grew progressively in semi-solid medium could not be induced to form cornified envelopes. The other five lines, which grew abortively, at best, in semisolid medium, formed envelopes under this condition but at subnormal rates. During anchorage deprivation the SCC lines became committed to differentiate much more slowly than normal, with t1/2's of 24--144 hr. SCC cells therefore possess at least a partial defect in the triggering of terminal differentiation. In vivo, such an alteration may permit malignant behavior by evading an important tissue-specific mechanism for limiting growth. In culture, the phenotype of increased survival in semi-solid medium may be used to detect and select malignantly transformed keratinocytes.

Citations

Sep 1, 1993·Journal of Cellular Physiology·R L Konger, T C Chan
Jan 1, 1996·Molecular Biology Reports·T T SunX R Wu
Jan 1, 1991·Archives of Dermatological Research·M F te PasM Ponec
Jan 1, 1983·Virchows Archiv. A, Pathological Anatomy and Histopathology·P MonaghanB Gusterson
Sep 1, 1985·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·G Serrero
Aug 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·M PonecJ Boonstra
Aug 1, 1989·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·M PonecM L Williams
Sep 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·A L Rubin, R H Rice
Oct 1, 1996·In Vitro Cellular & Developmental Biology. Animal·K JanssonA Haegerstrand
Oct 17, 1987·Biochimica Et Biophysica Acta·M PonecJ Boonstra
Nov 1, 1986·Experimental Cell Research·I KingA C Sartorelli
Jan 1, 1986·Advances in Enzyme Regulation·A C SartorelliM Reiss
Jan 1, 1986·Biochimica Et Biophysica Acta·S E Chang
May 10, 2005·Oral Oncology·Wenhui CaoMarshall R Posner
Jan 1, 1997·Pharmacology & Therapeutics·R E Scott
Aug 1, 1999·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A C GrayR H Clothier
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·K H KimE Fuchs
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·B L Allen-Hoffmann, J G Rheinwald
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T MasuiC C Harris
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R R WeichselbaumT J Ervin
Mar 1, 1988·The Journal of Cell Biology·P BoukampN E Fusenig
Sep 1, 1982·The Journal of Cell Biology·K AlitaloA Vaheri
Apr 1, 1993·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·H HaradaS Yasumoto
Sep 13, 2005·Cancer Cell International·Toni M RichterSteven B Scholnick
Aug 13, 2003·Biological & Pharmaceutical Bulletin·Ki-Young MoonYeong Shik Kim

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