PMID: 11922108Apr 2, 2002Paper

Defects in glycosylphosphatidylinositol (GPI) anchor synthesis activate Hog1 kinase and confer copper-resistance in Saccharomyces cerevisisae

Genes & Genetic Systems
Akio Toh-e, Tomoko Oguchi

Abstract

Las21/Gpi7 contains a heavy-metal-associated motif at its N-terminus. When this motif was disrupted by amino acid substitution, the cells acquired weak copper-resistance. We found that the previously isolated las21 mutants were strongly resistant to copper. Metallothionein is necessary for the expression of the copper-resistance of the las21 mutants. However, hyper-production of metallothionein is unlikely to be the cause of copper-resistance of the las21 mutants. Copper-sensitive mutants (collectively called Cus mutants) were isolated from the las21delta and characterized. One of the Cus genes was found to be PBS2, which encodes Hog1 MAP kinase kinase, indicating that the Hog1 MAP kinase pathway is needed for the expression of copper-resistance of the las21 mutants. As expected, the las21delta hog1delta strain was no longer copper-resistant. We found that Hog1 was constitutively activated in las21delta cells and in ssk1delta las21delta cells but not in sho1delta las21delta cells. Inactivation of either FSR2/MCD4 or MPC1/GPI13, both of which are involved in GPI anchor synthesis, like LAS21, caused a similar level of constitutive activation of Hog1 kinase and copper-resistance as found in the las21delta strain. The constitutive ...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jan 1, 1991·Methods in Enzymology·F Sherman
Jul 1, 1988·Molecular and Cellular Biology·D J Thiele
May 10, 1985·Science·D H HamerJ E Lemontt
Sep 1, 1965·Canadian Journal of Genetics and Cytology. Journal Canadien De Génétique Et De Cytologie·G LindegrenC C Lindegren
Jun 25, 1982·Journal of Molecular Biology·D T StinchcombR W Davis
Nov 24, 1995·The Journal of Biological Chemistry·K KampfenkelM Van Montagu
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·D S YuanR D Klausner
Mar 9, 1995·Nature·S M LiaoR A Young
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·A M Neiman, I Herskowitz
Jan 7, 1997·Proceedings of the National Academy of Sciences of the United States of America·Z S LiP A Rea
Apr 4, 1997·The Journal of Biological Chemistry·S J LinV C Culotta
Nov 5, 1997·Science·R A PufahlT V O'Halloran
Jan 27, 1998·The Journal of Biological Chemistry·Z ZhuD J Thiele
Jun 10, 1998·Microbiology and Molecular Biology Reviews : MMBR·F Banuett
Jan 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·C RensingB P Rosen
Mar 29, 2000·Proceedings of the National Academy of Sciences of the United States of America·Z WeissmanD Kornitzer

❮ Previous
Next ❯

Citations

Nov 5, 2003·Molecular Biology of the Cell·Sean M O'Rourke, Ira Herskowitz
Sep 16, 2005·Molecular Biology of the Cell·Yoshiko MurakamiTaroh Kinoshita
Jan 24, 2009·Proceedings of the National Academy of Sciences of the United States of America·André X C N ValenteYuan Gao
Oct 3, 2012·Genetics·Haruo Saito, Francesc Posas
Jul 12, 2002·Yeast
Jul 12, 2018·Molecular Microbiology·Telma S MartinsClara Pereira
Dec 12, 2021·Critical Reviews in Microbiology·Hajar YaakoubNicolas Papon

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.