PMID: 9529061Apr 7, 1998Paper

Defects in type III secretion correlate with internalization of Pseudomonas aeruginosa by epithelial cells

Infection and Immunity
A R HauserJ N Engel

Abstract

Previous characterization of Pseudomonas aeruginosa clinical isolates has demonstrated an inverse correlation between cytotoxicity and internalization by epithelial cells. To further investigate this relationship, we tested PA103, a cytotoxic P. aeruginosa strain, and 33 isogenic noncytotoxic transposon mutants for internalization by Madin-Darby canine kidney cells. The majority of the mutants were not internalized, demonstrating that an inverse correlation between cytotoxicity and bacterial uptake by epithelial cells is not absolute. Six of the noncytotoxic mutants, however, demonstrated measurable levels of internalization by standard aminoglycoside exclusion assays even though internalization of wild-type strain PA103 was not detectable. All six had evidence of protein secretion defects involving two proteins, a 40-kDa protein and a 32-kDa protein. These proteins, designated PepB (for Pseudomonas exoprotein B) and PepD, respectively, each had characteristics of type III transported proteins. In addition, nucleotide sequencing studies demonstrated that PepB and PepD are homologs of YopB and YopD, respectively, type III secreted proteins of Yersinia spp. necessary for the translocation of effector molecules into the cytoplasmi...Continue Reading

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Citations

Apr 24, 2020·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Jimyeong HaKyoung-Hee Choi
Feb 24, 2001·Cellular Microbiology·J D Ernst
Feb 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Stefan PukatzkiJohn J Mekalanos
May 30, 2002·American Journal of Respiratory Cell and Molecular Biology·Chieh-Liang WuJeffrey A Kazzaz
May 9, 2002·British Medical Bulletin·C Anthony Hart, Craig Winstanley
Dec 22, 1999·Infection and Immunity·B A CowellS M Fleiszig
Feb 24, 2001·Cellular Microbiology·B I KazmierczakJ N Engel
Nov 24, 2004·Infection and Immunity·Ciara M Shaver, Alan R Hauser
Dec 12, 2007·The Journal of Experimental Medicine·Fayyaz S SutterwalaRichard A Flavell
Feb 20, 2007·Journal of Bacteriology·Chantal SosciaSophie Bleves
Oct 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Sasha H Shafikhani, Joanne Engel
Oct 29, 2014·Antimicrobial Agents and Chemotherapy·Julien M BuyckFrançoise Van Bambeke
Feb 8, 2005·American Journal of Respiratory and Critical Care Medicine·Ruxana T SadikotAlice S Prince
Aug 10, 2000·Proceedings of the National Academy of Sciences of the United States of America·J K IchikawaS Lory
Nov 29, 2005·Cellular Microbiology·A M SalibaM C Plotkowski
May 1, 2013·Infection and Immunity·Sushmita MustafiM Alejandro Barbieri
Jul 30, 2008·Infection and Immunity·Maureen H DiazAlan R Hauser

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