Defense of zebrafish embryos against Streptococcus pneumoniae infection is dependent on the phagocytic activity of leukocytes

Developmental and Comparative Immunology
Samuli RouniojaMika Rämet

Abstract

Severe community acquired pneumonia caused by Streptococcus pneumoniae is the most common cause of death from infection in developing countries. Serotype specific conjugate vaccines have decreased the incidence of invasive infections, but at the same time, disease due to non-vaccine serotypes have increased. New insights into host immune mechanisms against pneumococcus may provide better treatment and prevention strategies. Zebrafish is an attractive vertebrate model for studying host immune responses and infection biology. Here we show that an intravenous challenge with pneumococcus infects zebrafish embryos leading to death in a dose dependent manner. Survival rates correlate with the bacterial burden in the embryos. The production of proinflammatory cytokines is induced in zebrafish after pneumococcal exposure. Importantly, morpholino treated embryos lacking either myeloid cells or the ability to phagocytose bacteria have lowered survival rates compared to wild type embryos after pneumococcal challenge. These data suggest that the survival of zebrafish embryos upon intravenous infection with S. pneumoniae is dependent on the clearance of the bacteria by phagocytosing cells. Additionally, we demonstrate that mutant pneumococc...Continue Reading

References

May 11, 1995·The New England Journal of Medicine·Elaine TuomanenH R Masure
May 31, 2001·Microbiology and Molecular Biology Reviews : MMBR·M J Jedrzejas
Dec 12, 2002·Microbes and Infection·Jeffrey A YoderGary W Litman
Dec 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jeremy S BrownMarina Botto
Apr 3, 2003·Immunological Reviews·Karen BadourKatherine A Siminovitch
Jul 12, 2003·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Victor L YuInternational Pneumococcal Study Group
Dec 23, 2003·Molecular Immunology·Cyril JaultJohanna Chluba
Apr 23, 2004·Infection and Immunity·Francesco IannelliGianni Pozzi
May 26, 2004·Acta Tropica·Jesse D Miller, Melody N Neely
Sep 24, 2004·Trends in Microbiology·Astrid M van der SarWilbert Bitter
Oct 23, 2004·Clinical and Experimental Immunology·R A HirstP W Andrew
Mar 9, 2005·Developmental and Comparative Immunology·Meagan E PressleyCarol H Kim
Aug 10, 2005·The Journal of Infectious Diseases·Andreas SandgrenB Henriques-Normark
Sep 27, 2005·Microbes and Infection·Gavin K Paterson, T J Mitchell
Feb 17, 2006·Proceedings of the National Academy of Sciences of the United States of America·M A BarocchiB Henriques-Normark
Mar 23, 2006·Infection and Immunity·Astrid M van der SarAnnemarie H Meijer
May 19, 2006·Proceedings of the National Academy of Sciences of the United States of America·Judy K MoronaJames C Paton
Nov 8, 2006·Infection and Immunity·Aaron L NelsonJeffrey N Weiser
Sep 14, 2007·Molecular Microbiology·Aaron L NelsonB Henriques-Normark
Nov 17, 2007·Cell Host & Microbe·Hilary ClayLalita Ramakrishnan
Jun 11, 2008·Cell Host & Microbe·Ken J IshiiShizuo Akira
Aug 2, 2008·Science·Horst von BernuthJean-Laurent Casanova
Jan 27, 2009·Infection and Immunity·Anne E ClatworthyDeborah T Hung

Citations

Oct 24, 2012·Infection and Immunity·Elizabeth A HarvieAnna Huttenlocher
Sep 5, 2013·Infection and Immunity·Tomasz K PrajsnarStéphane Mesnage
Nov 10, 2013·PloS One·Barbara TaskinenVesa P Hytönen
Aug 8, 2012·Developmental and Comparative Immunology·Hayley PattersonMika Rämet
Aug 29, 2012·Acta Paediatrica·Olli LohiMika Rämet
Jun 23, 2015·Scandinavian Journal of Immunology·Anni Saralahti, Mika Rämet
Oct 1, 2013·Developmental and Comparative Immunology·Anni SaralahtiSamuli Rounioja
Feb 24, 2015·Frontiers in Microbiology·Yamilé López HernándezIsidre Gibert
Aug 21, 2016·Journal of Neuroinflammation·Kin Ki JimChristina M J E Vandenbroucke-Grauls
Apr 12, 2019·Frontiers in Microbiology·Hilger JagauSimone Bergmann
Jul 11, 2015·Médecine sciences : M/S·Audrey BernutLaurent Kremer

Related Concepts

Embryonic Structures, Nonmammalian
Immunofluorescence Microscopy
Phagocytosis
Pneumococcal Infections
Poly(A) Tail
Streptococcus pneumoniae
Zebrafish
Insertion Mutation
Reverse Transcriptase Polymerase Chain Reaction
Virulence Factors

Related Feeds

Bacterial Pneumonia

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Bacterial Pneumonia (ASM)

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.