Defensin from disk abalone Haliotis discus discus: molecular cloning, sequence characterization and immune response against bacterial infection

Fish & Shellfish Immunology
Mahanama De ZoysaJehee Lee

Abstract

Gene-encoded antimicrobial peptides (AMPs) serve a major role in host defense systems against pathogens. In this study, cDNA of a new mollusk defensin was identified from a normalized cDNA library constructed from whole tissues of disk abalone. Abalone defensin peptide (pro-defensin) has a 198-bp coding sequence comprised of a putative 66 amino acids with a mature defensin consisting of 48 amino acid residues. The presence of an invertebrate defensin family domain, an arrangement of six cysteine residues and their disulfide linkage in C(1)-C(4), C(2)-C(5) and C(3)-C(6) form, an alpha helix in three-dimensional structure and a phylogenetic relationship suggests that abalone defensin could be a new member of the invertebrate defensin family, and related to arthropod defensins. In non-stimulated abalone, defensin transcripts were constitutively expressed in all examined tissues including hemocytes, gills, mantle, muscle, digestive tract and hepatopancreas. It was observed that abalone defensin transcripts were significantly induced in hemocytes, gills and digestive tract at different time intervals after infection by pathogenic bacteria mixture containing Vibrio alginolyticus, Vibrio parahemolyticus and Lysteria monocytogenes. Our...Continue Reading

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Citations

Mar 30, 2013·Developmental and Comparative Immunology·Erick Garcia-GarciaVictor Mulero
May 4, 2011·The Journal of Biological Chemistry·Jeremie Vidal-DupiolGuillaume Mitta
May 19, 2011·Yi chuan = Hereditas·Lan-Bao FuWei-Hua Liu
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Jan 28, 2012·Fish & Shellfish Immunology·Lei TengShicui Zhang
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Aug 16, 2011·Fish & Shellfish Immunology·Qian RenKe-Ping Chen
Sep 13, 2015·Fish & Shellfish Immunology·Qing WangJianmin Zhao
Dec 19, 2012·Fish & Shellfish Immunology·Zhi LiaoWang Shen
Dec 3, 2014·Developmental and Comparative Immunology·Jun ZhuangLianhui Xie
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Nov 21, 2019·Developmental and Comparative Immunology·Gabriela ContrerasMichael Wink

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