Defensome against toxic diatom aldehydes in the sea urchin Paracentrotus lividus.

PloS One
Vincenzo MarroneMaria Costantini

Abstract

Many diatom species produce polyunsaturated aldehydes, such as decadienal, which compromise embryonic and larval development in benthic organisms. Here newly fertilized Paracentrotus lividus sea urchins were exposed to low concentration of decadienal and the expression levels of sixteen genes, implicated in a broad range of functional responses, were followed by Real Time qPCR in order to identify potential decadienal targets. We show that at low decadienal concentrations the sea urchin Paracentrotus lividus places in motion different classes of genes to defend itself against this toxic aldehyde, activating hsp60 and two proteases, hat and BP10, at the blastula stage and hsp56 and several other genes (14-3-3ε, p38 MAPK, MTase, and GS) at the prism stage. At this latter stage all genes involved in skeletogenesis (Nec, uni, SM50 and SM30) were also down-expressed, following developmental abnormalities that mainly affected skeleton morphogenesis. Moreover, sea urchin embryos treated with increasing concentrations of decadienal revealed a dose-dependent response of activated target genes. Finally, we suggest that this orchestrated defense system against decadienal represents part of the chemical defensome of P. lividus affording pr...Continue Reading

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Citations

Mar 18, 2016·Toxicological Sciences : an Official Journal of the Society of Toxicology·Stefano VarrellaMaria Costantini
Aug 27, 2016·Scientific Reports·Nadia RuoccoLuigia Santella
Apr 25, 2017·Ecotoxicology and Environmental Safety·Nadia RuoccoMaria Costantini
Feb 26, 2016·PloS One·Stefano VarrellaMaria Costantini
Jan 26, 2020·International Journal of Molecular Sciences·Roberta EspositoMaria Costantini
Jul 28, 2020·International Journal of Molecular Sciences·Christian GalassoGiovanna Romano
Jan 11, 2020·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Mirella VazzanaGiuseppa Buscaino
Sep 28, 2021·Frontiers in Microbiology·Shiri Graff van CreveldAssaf Vardi

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Datasets Mentioned

BETA
AJ249625

Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

GraphPad
REST ( Relative Expression Software Tool )
GraphPad Prism
[UNK]

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