PMID: 9548269Apr 21, 1998Paper

Deferoxamine arrests in vitro the proliferation of porcine hepatocyte in G1 phase of the cell cycle

Liver
N ChenoufiO Loréal

Abstract

Iron is required for cell proliferation of all living species. Moreover, iron excess may be involved in the development of hepatocellular carcinoma. In this study we analyzed the effects of deferoxamine, an iron chelator, on normal porcine hepatocyte proliferation. We confirmed that hepatocytes isolated from young pigs proliferate in the presence of insulin and fetal calf serum as shown by [3H] methyl-thymidine incorporation, presence of mitotic figures and increase in cell number. This was paralleled by nuclear expression of p34cdc2 and its associated histone H1 kinase activity. In the presence of deferoxamine, [3H] methyl-thymidine incorporation, expression of nuclear proteins (p34cdc2 and PCNA) and H1 kinase activity were drastically reduced. In addition, in contrast with control cultures, cells in S-phase were not detected by flow cytometry. These data suggest that iron chelation by deferoxamine can arrest the progression of porcine hepatocytes in the G1 phase of the cell cycle.

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Citations

Sep 1, 2006·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·François GaboriauGérard Lescoat
Nov 4, 2009·Journal of Enzyme Inhibition and Medicinal Chemistry·Pascal RougePascal Sonnet
Jun 16, 2010·Journal of Enzyme Inhibition and Medicinal Chemistry·Laurent LatxaguePascal Sonnet
Jun 17, 2009·Journal of Drug Targeting·Allan LangloisSeverine Sigrist
Nov 5, 1999·Carcinogenesis·K Kramer-SticklandG T Bowden
Aug 22, 2006·Journal of Enzyme Inhibition and Medicinal Chemistry·Viviane Silva PiresPascal Sonnet
May 6, 2016·Annual Review of Nutrition·Marie Balslev BackeThomas Mandrup-Poulsen

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