Deficiency in Fpr2 results in reduced numbers of Lin- cKit+ Sca1+ myeloid progenitor cells

The Journal of Biological Chemistry
Keqiang ChenJi Ming Wang

Abstract

The Lin-c-Kit+ Sca-1+ cell population in the bone marrow (BM) serves as the direct precursor for differentiation of myeloid cells. In this study, we report that deficiency in Fpr2, a G protein-coupled chemoattractant receptor in mice, is associated with reduced BM nucleated cells, including CD31+Ly6C+ (granulocytes and monocytes), CD31-/Ly6Cint (granuloid cells), and CD31-/Ly6Chigh (predominantly monocytes) cells. In particular, the number of Lin-c-Kit+Sca-1+ (LKS) cells was reduced in Fpr2-/- mouse BM. This was supported by observations of the reduced incorporation of intraperitoneally injected bromodeoxyuridine by cells in the c-Kit+ population from Fpr2-/- mouse BM. Purified c-Kit+ cells from Fpr2-/- mice showed reduced expansion when cultured in vitro with stem cell factor (SCF). SCF/c-Kit-mediated phosphorylation of P38, STAT1, Akt (Thr-308), and Akt (Ser-473) was also significantly reduced in c-Kit+ cells from Fpr2-/- mice. Furthermore, Fpr2 agonists enhanced SCF-induced proliferation of c-Kit+ cells. Colony-forming unit assays revealed that CFU-granulocyte-macrophage formation of BM cells from Fpr2-/- mice was significantly reduced. After heat-inactivated bacterial stimulation in the airway, the expansion of c-kit+ Sca-1...Continue Reading

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Citations

Jun 13, 2019·Stem Cells Translational Medicine·Mario RomanoAntonio Recchiuti
Aug 10, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sebastian SchloerUrsula Rescher
Dec 1, 2020·Technology in Cancer Research & Treatment·Cuimeng TianJi Ming Wang

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