Deficiency of the Complement Component 3 but Not Factor B Aggravates Staphylococcus aureus Septic Arthritis in Mice

Infection and Immunity
Manli NaTao Jin

Abstract

The complement system plays an essential role in the innate immune response and protection against bacterial infections. However, detailed knowledge regarding the role of complement in Staphylococcus aureus septic arthritis is still largely missing. In this study, we elucidated the roles of selected complement proteins in S. aureus septic arthritis. Mice lacking the complement component 3 (C3(-/-)), complement factor B (fB(-/-)), and receptor for C3-derived anaphylatoxin C3a (C3aR(-/-)) and wild-type (WT) control mice were intravenously or intra-articularly inoculated with S. aureus strain Newman. The clinical course of septic arthritis, as well as histopathological and radiological changes in joints, was assessed. After intravenous inoculation, arthritis severity and frequency were significantly higher in C3(-/-)mice than in WT controls, whereas fB(-/-)mice displayed intermediate arthritis severity and frequency. This was in accordance with both histopathological and radiological findings. C3, but not fB, deficiency was associated with greater weight loss, more frequent kidney abscesses, and higher bacterial burden in kidneys. S. aureus opsonized with C3(-/-)sera displayed decreased uptake by mouse peritoneal macrophages compa...Continue Reading

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Citations

Nov 16, 2019·Scientific Reports·Tao JinAbukar Ali
May 15, 2020·Scientific Reports·Majd MohammadTao Jin
Mar 1, 2020·BMC Infectious Diseases·Berglind BergmannInger Gjertsson
Aug 10, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Srijana PokhrelPamela R Hall
May 23, 2017·Frontiers in Cellular and Infection Microbiology·Giampiero PietrocolaPietro Speziale
Feb 6, 2021·Frontiers in Immunology·Grace R PidwillSimon J Foster

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Methods Mentioned

BETA
X-ray
lavage
ELISA
flow cytometry

Software Mentioned

IDEAS
NRECON
FCAP array

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