Deficient alpha-smooth muscle actin as a cause of functional intestinal obstruction in childhood.

Pediatric Surgery International
Anne-Marie O' DonnellPrem Puri

Abstract

Most functional intestinal obstruction in childhood is due to defects in the enteric innervation. Functional intestinal obstruction due to smooth muscle disorders is rare. The aim of this study was to describe four patients with functional intestinal obstruction where the only histopathological abnormality was deficient, alpha-smooth muscle actin (alpha-SMA) in the circular muscle of their small bowel. Resected intestinal segments from four children with functional intestinal obstruction were either snap frozen or embedded in paraffin wax, and transversely sectioned. Sections were examined by routine H&E staining, AChE and NADPH-diaphorase histochemistry, and immunohistochemistry using PGP9.5 and alpha-SMA antibodies. Normal age-matched tissue was used as controls. All four patients demonstrated normal innervation in the small and large bowel. We observed striking differences in the expression of alpha-SMA within the intestinal muscle layers in all four patients. There was a markedly decreased level of alpha-SMA in the smooth muscle compared to the longitudinal muscle in the small bowel in each patient. All other regions of the intestine examined showed normal levels of alpha-SMA, with similar levels expressed in both the circu...Continue Reading

References

Dec 1, 1992·Histopathology·V V SmithR J Nicholls
Sep 1, 1987·Gastroenterology·S Krishnamurthy, M D Schuffler
Jun 1, 1995·Microscopy Research and Technique·M H Stromer
Mar 1, 1964·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M J KARNOVSKY, L ROOTS
Jun 15, 2006·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·T WedelJ-M Vanderwinden

❮ Previous
Next ❯

Citations

Dec 24, 2009·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·Reka SzigetiNina Tatevian
Apr 25, 2013·Journal of Ethnopharmacology·Siddig Ibrahim AbdelwahabOnn Haji Hashim
Sep 23, 2011·The Indian Journal of Surgery·Santoshkumar Nagnath DeshmukhShweta P Shende
Dec 13, 2012·Advances in Anatomic Pathology·Nunzia BernardiniAmelio Dolfi
Mar 2, 2016·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Katherine GurdzielDeborah L Gumucio

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.