PMID: 15381722Sep 24, 2004Paper

Deficient cell proliferation in palatal shelf mesenchyme of CL/Fr mouse embryos

Journal of Dental Research
Y SasakiY Taya

Abstract

How secondary palate formation is affected in the cleft lip genotype remains poorly understood. The purpose of this study was to analyze regional patterns of cell proliferation in CL/Fr mouse embryos with or without cleft lip. Pairs of palatal shelves were dissected at E13.5 from CL/Fr normal embryos (CL/Fr-N), CL/Fr embryos with bilateral cleft lip (CL/Fr-BCL), and a control strain of C57BL embryos (C57BL). The explants were examined histologically after 48 hrs of organ culture. Cell kinetics for proliferation in the palatal shelves was examined at E13.5 by the bromodeoxyuridine method in vivo. The CL/Fr-BCL palates fused as well as the CL/Fr-N palates in vitro. There were inter-group differences in the absolute number of BrdU-positive cells and the ratio of positive/(positive+negative) cells in the palate's mesenchyme (C57BL > CL/Fr-N > CL/Fr-BCL) and epithelium (C57BL > CL/Fr-N = CL/Fr-BCL). These findings indicate that a cleft palate follows reduced cell proliferation of secondary palatal mesenchyme in CL/Fr mice.

References

Jul 1, 1990·The Cleft Palate Journal·R K WyseK D Blake
Feb 1, 1963·Developmental Biology·D G Trasler, F C Fraser
May 1, 1982·The American Journal of Anatomy·G MillicovskyM C Johnston
Mar 1, 1997·The Cleft Palate-craniofacial Journal : Official Publication of the American Cleft Palate-Craniofacial Association·M NagataA Oguro
May 1, 1997·The Cleft Palate-craniofacial Journal : Official Publication of the American Cleft Palate-Craniofacial Association·S KadowakiT Tanimura
Jul 1, 1997·The Cleft Palate-craniofacial Journal : Official Publication of the American Cleft Palate-Craniofacial Association·K NonakaM Nakata
Mar 3, 1998·The Cleft Palate-craniofacial Journal : Official Publication of the American Cleft Palate-Craniofacial Association·S KadowakiT Tanimura
Feb 19, 2000·Teratogenesis, Carcinogenesis, and Mutagenesis·T N TakagiM Yasuda

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Citations

Jul 15, 2009·The International Journal of Developmental Biology·Dennis R WarnerM Michele Pisano
Nov 12, 2013·Congenital Anomalies·Yasunori SasakiTaku Fujiwara
Jan 9, 2008·Birth Defects Research. Part A, Clinical and Molecular Teratology·Diana M Juriloff, Muriel J Harris

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