Deficient natural killer function in patients receiving immunosuppressive drugs: analysis at the cellular level

Cellular Immunology
E DupontJ Wybran

Abstract

Renal transplant recipients receiving low maintenance immunosuppression (azathioprine, 2 mg/kg/day, and prednisolone, 10 mg/day), tolerating their transplants well, and without viral infection disclose a profound depression of NK activity as assessed by 51Cr-release assay. By combining the analysis of the different steps of cytolysis with the agarose single-effector assays and the estimation of circulating large granular lymphocytes (LGL), the defect is shown to be due to a significant decrease of the number of NK cells capable of binding (% target-binding cells 2.0 +/- 0.3 versus 5.7 +/- 0.7 in normals, P less than 0.001) and killing (% cytotoxic target-binding cells 12.4 +/- 1.9 versus 22.0 +/- 0.5 in normals, P less than 0.001) of targets. There is also a significant reduction (P less than 0.001) of both percentages (1.0 +/- 0.2 versus 3.3 +/- 0.4 in normals) and absolute values (9.8 +/- 2.4 versus 62.3 +/- 8.0/microliters in normals) of LGL. These observations indicate that depressed NK activity is due mostly to depletion of NK cells. Functional impairment of NK cells can also be involved. Lack of direct in vitro effects of drugs (6-mercaptopurine, hydrocortisone, and methylprednisolone) at concentrations likely to be reach...Continue Reading

References

Oct 1, 1982·Proceedings of the Society for Experimental Biology and Medicine·T C CesarioJ G Tilles
Mar 1, 1981·Transplantation·E GoulmyJ J van Rood
Mar 1, 1981·The Journal of Experimental Medicine·M Ullberg, M Jondal

Citations

Jan 1, 1988·International Journal of Immunopharmacology·M P FuggettaE Bonmassar
Jul 1, 1995·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·M BüttnerK Heinritzi
Sep 1, 1987·European Journal of Cancer & Clinical Oncology·A JermyR C Rees
Jan 1, 1987·Immunopharmacology and Immunotoxicology·L DénesL Kisfaludy

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