Defined Geldrop Cultures Maintain Neural Precursor Cells

Scientific Reports
Steffen VoglerGerd Kempermann

Abstract

Distinct micro-environmental properties have been reported to be essential for maintenance of neural precursor cells (NPCs) within the adult brain. Due to high complexity and technical limitations, the natural niche can barely be studied systematically in vivo. By reconstituting selected environmental properties (adhesiveness, proteolytic degradability, and elasticity) in geldrop cultures, we show that NPCs can be maintained stably at high density over an extended period of time (up to 8 days). In both conventional systems, neurospheres and monolayer cultures, they would expand and (in the case of neurospheres) differentiate rapidly. Further, we report a critical dualism between matrix adhesiveness and degradability. Only if both features are functional NPCs stay proliferative. Lastly, Rho-associated protein kinase was identified as part of a pivotal intracellular signaling cascade controlling cell morphology in response to environmental cues inside geldrop cultures. Our findings demonstrate that simple manipulations of the microenvironment in vitro result in an important preservation of stemness features in the cultured precursor cells.

References

Feb 1, 1994·The Journal of Cell Biology·S M Frisch, H Francis
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J RayF H Gage
May 5, 1997·FEBS Letters·M OkabeY Nishimune
Jan 8, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Z ChangA Friedl
Feb 2, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D M Ornitz
Dec 4, 2003·Brain Research·Alessandra BezEugenio Agostino Parati
Apr 23, 2005·Nature Methods·Brent A Reynolds, Rodney L Rietze
Jul 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·A Katrin GoetzDennis A Steindler
Feb 20, 2007·Molecular Neurobiology·Josephine B Jensen, Malin Parmar
Aug 4, 2007·Experimental Cell Research·Kiran BhadrirajuChristopher S Chen
Sep 22, 2007·Biomacromolecules·Martin EhrbarMatthias P Lutolf
Nov 13, 2008·PLoS Biology·Sebastian JessbergerFred H Gage
May 7, 2009·Stem Cells·Eric M WexlerDaniel H Geschwind
Aug 19, 2009·PLoS Biology·Karine LoulierCharles Ffrench-Constant
Nov 27, 2009·Nature·Matthias P LutolfHelen M Blau
May 25, 2010·Developmental Cell·Stephanie L Gupton, Frank B Gertler
Jan 20, 2011·Biophysical Journal·M EhrbarM P Lutolf
Jul 19, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·A ZierisC Werner
Oct 11, 2011·Nature Methods·Samy GobaaMatthias P Lutolf
Feb 22, 2012·The Journal of Biological Chemistry·Sachiko Murase, Ronald D McKay
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Aug 3, 2012·Nature Cell Biology·Peter FriedlJeffrey E Segall
Apr 12, 2013·Advanced Materials·Mikhail V TsurkanCarsten Werner
Mar 19, 2014·Nature Materials·Chun YangKristi S Anseth
Mar 19, 2014·Journal of Visualized Experiments : JoVE·Tara L Walker, Gerd Kempermann
Jul 17, 2014·Nature Communications·A RangaM P Lutolf
Dec 3, 2014·Progress in Neurobiology·Amy M HopkinsDavid L Kaplan
Jan 12, 2016·Nature Materials·Massimiliano CaiazzoMatthias P Lutolf

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Citations

Apr 6, 2019·Nature Biomedical Engineering·Carsten Werner
Mar 19, 2021·Neurochemistry International·Dimitri EigelBen Newland
Sep 30, 2021·Science Advances·Christopher RathnamKi-Bum Lee

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