Oct 23, 1989

Defined monoclonal antibodies to Escherichia coli beta-galactosidase as a tool for characterisation of recombinant expression products

FEBS Letters
M Bröker, H P Harthus


Mouse monoclonal antibodies were prepared against beta-galactosidase (EC of Escherichia coli. The binding sites of these monoclonal antibodies within the beta-galactosidase molecule were estimated by immunoblot analyses to various defined peptide regions of beta-galactosidase, encoded by expression plasmids. Monoclonal antibodies were characterised, which either bind to the amino-terminal or to the carboxy-terminal region or to an internal section of beta-galactosidase. These defined monoclonal antibodies were shown to be a useful tool for characterisation of beta-galactosidase fusion proteins expressed in Escherichia coli.

Mentioned in this Paper

Monoclonal Antibodies
Immunoblotting, Reverse
Alkalescens-Dispar Group
Mice, Inbred BALB C
Chimeric Proteins, Recombinant
Antigenic Specificity
Escherichia Coli Infections
Gene Expression
Hybrid Proteins, Recombinant

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