PMID: 18408352Apr 15, 2008Paper

Defined system for in vitro production of porcine embryos using a single basic medium

The Journal of Reproduction and Development
Koji YoshiokaAkira Onishi

Abstract

We have previously indicated that porcine blastocysts can be produced by in vitro fertilization (IVF) and culture (IVC) in chemically defined porcine gamete medium (PGM) and porcine zygote medium (PZM)-5, respectively, In the present study, the effects of basic media and macromolecular components on in vitro maturation (IVM) were investigated to develop a defined system for in vitro embryo production using a single basic medium through IVM, IVF and IVC. Porcine immature oocytes were matured in porcine oocyte medium (POM) or modified North Carolina State University (mNCSU) 37, which were supplemented with either 10% (v/v) porcine follicular fluid (pFF) or 3 mg/ml polyvinyl alcohol (PVA) as a macromolecular component (designated POM+pFF, POM+PVA, mNCSU37+pFF and mNCSU37+PVA). In the maturation with mNCSU37+PVA, the percentages of oocytes that reached the metaphase II stages were significantly lower than those in the other treatments. Following IVM with the above media, oocytes were treated with an electrical stimulus and cycloheximide for parthenogenetic activation and were cultured in PZM-5 for 5 days. The rates of cleavage and blastocyst formation of parthenogenetic oocytes were significantly lowered for maturation with mNCSU37...Continue Reading

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Citations

Jul 9, 2010·Obstetrics and Gynecology International·Ning WangFan Jin
Mar 23, 2011·The Journal of Reproduction and Development·Koji Yoshioka
Aug 2, 2011·The Journal of Reproduction and Development·Kenji Momozawa, Yoshinori Fukuda
Apr 9, 2011·Zygote : the Biology of Gametes and Early Embryos·Zubing CaoYunhai Zhang
Nov 20, 2015·The Journal of Reproduction and Development·Chie SuzukiKoji Yoshioka
Nov 28, 2013·Theriogenology·Christopher G Grupen
Nov 6, 2012·Fertility and Sterility·Koji MatsuuraHiroaki Funahashi
Jun 16, 2012·Animal Reproduction Science·Wei ZhangXu Zhou
Mar 2, 2012·Animal Reproduction Science·Koji YoshiokaChie Suzuki
Mar 11, 2015·The Journal of Reproduction and Development·Rukmali AthurupanaHiroaki Funahashi
Dec 3, 2014·Zygote : the Biology of Gametes and Early Embryos·Erika WakizoeYasuhiro Tsuzuki
Apr 24, 2013·Animal Science Journal = Nihon Chikusan Gakkaihō·Masahiko HiroseNaomi Kashiwazaki
May 28, 2011·Animal Science Journal = Nihon Chikusan Gakkaihō·Thanh Quang Dang-NguyenTakashi Nagai
Jan 11, 2013·Animal Science Journal = Nihon Chikusan Gakkaihō·Rentsenkhand SambuuTakeshige Otoi
May 4, 2013·Animal Science Journal = Nihon Chikusan Gakkaihō·Thanh Quang Dang-NguyenTakashi Nagai
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Jun 7, 2015·Theriogenology·Yubyeol JeonSang-Hwan Hyun
Feb 23, 2013·The Journal of Reproduction and Development·Budiyanto AgungTakashi Nagai
May 20, 2015·Developmental Biology·Satoshi SugimuraRobert B Gilchrist
May 11, 2013·Zygote : the Biology of Gametes and Early Embryos·Michiko NoguchiKazuhiro Kikuchi
Oct 21, 2016·The Journal of Reproduction and Development·Ruth AppeltantKazuhiro Kikuchi
Dec 14, 2016·Zygote : the Biology of Gametes and Early Embryos·Elisa C S SantosThomaz Lucia

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