Defining a role for lung function associated gene GSTCD in cell homeostasis

Respiratory Research
Amanda P HenryIan Sayers

Abstract

Genome wide association (GWA) studies have reproducibly identified signals on chromosome 4q24 associated with lung function and COPD. GSTCD (Glutathione S-transferase C-terminal domain containing) represents a candidate causal gene in this locus, however little is currently known about the function of this protein. We set out to further our understanding of the role of GSTCD in cell functions and homeostasis using multiple molecular and cellular approaches in airway relevant cells. Recombinant expression of human GSTCD in conjunction with a GST activity assay did not identify any enzymatic activity for two GSTCD isoforms questioning the assignment of this protein to this family of enzymes. Protein structure analyses identified a potential methyltransferase domain contained within GSTCD, with these enzymes linked to cell viability and apoptosis. Targeted knockdown (siRNA) of GSTCD in bronchial epithelial cells identified a role for GSTCD in cell viability as proliferation rates were not altered. To provide greater insight we completed transcriptomic analyses on cells with GSTCD expression knocked down and identified several differentially expressed genes including those implicated in airway biology; fibrosis e.g. TGFBR1 and infl...Continue Reading

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Citations

May 8, 2020·Clinical Reviews in Allergy & Immunology·Tesfaye B MershaGurjit K Khurana Hershey

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Methods Mentioned

BETA
Assay
transfection
RNA-seq
RNA seq
biopsies
RNAseq

Software Mentioned

FastQC©
Phyre2
CyQuant
TASSER
Cufflinks suite
GTex
Cuffdiff
- TASSER
Cufflinks
GraphPad Prism

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