Defining early events of Epstein-Barr virus (EBV) infection in immortalized nasopharyngeal epithelial cells using cell-free EBV infection

The Journal of General Virology
Pei Shin PangWen Deng

Abstract

Epstein-Barr virus (EBV) infection is strongly associated with nasopharyngeal carcinoma, a common cancer in Southeast Asia and certain regions of Africa. However, the dynamics of EBV episome maintenance in infected nasopharyngeal epithelial (NPE) cells remain largely undefined. Here, we report the establishment of a highly efficient cell-free EBV infection method for NPE cells. By using this method, we have defined some of the dynamic events involved in the early stage of EBV infection in NPE cells. We report, for the first time, a rapid loss of EBV copies from infected NPE cells during the first 12-72 h post-infection. The rate of EBV loss slowed at later stages of infection. Live cell imaging revealed that the freshly infected NPE cells were delayed in entry into mitosis compared with uninfected cells. Freshly infected NPE cells transcribed significantly higher levels of lytic EBV genes BZLF1 and BMRF1 yet significantly lower levels of EBER1/2 than stably infected NPE cells. Notably, there were very low or undetectable levels of protein expressions of EBNA1, LMP1, Zta and Rta in freshly infected NPE cells, whereas EBNA1 and LMP1 proteins were readily detected in stable EBV-infected NPE cells. The kinetics of EBV loss and the ...Continue Reading

References

Dec 1, 1991·Molecular and Cellular Biology·V Dhar, C L Schildkraut
Jun 8, 2001·Molecular and Cellular Biology·E R Leight, B Sugden
Dec 25, 2002·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Wen DengAnnie L M Cheung
Oct 29, 2004·Nature Reviews. Cancer·Lawrence S Young, Alan B Rickinson
Jan 3, 2006·Transplantation Proceedings·M AyatollahiA Samsami
May 12, 2006·International Journal of Cancer. Journal International Du Cancer·H M LiS W Tsao
Jan 22, 2010·International Journal of Cancer. Journal International Du Cancer·Chi Man TsangSai Wah Tsao
Nov 20, 2012·Proceedings of the National Academy of Sciences of the United States of America·Chi Man TsangSai Wah Tsao
Nov 7, 2013·Nature Reviews. Microbiology·Paul M Lieberman
Oct 15, 2014·Proceedings of the National Academy of Sciences of the United States of America·Rachel M TempleClare E Sample

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Citations

Oct 14, 2020·The Journal of International Medical Research·Yanming WuLei Zhang

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