Defining glycoprotein cancer biomarkers by MS in conjunction with glycoprotein enrichment

Biomarkers in Medicine
Ehwang Song, Yehia Mechref

Abstract

Protein glycosylation is an important and common post-translational modification. More than 50% of human proteins are believed to be glycosylated to modulate the functionality of proteins. Aberrant glycosylation has been correlated to several diseases, such as inflammatory skin diseases, diabetes mellitus, cardiovascular disorders, rheumatoid arthritis, Alzheimer's and prion diseases, and cancer. Many approved cancer biomarkers are glycoproteins which are not highly abundant proteins. Therefore, effective qualitative and quantitative assessment of glycoproteins entails enrichment methods. This chapter summarizes glycoprotein enrichment methods, including lectin affinity, immunoaffinity, hydrazide chemistry, hydrophilic interaction liquid chromatography, and click chemistry. The use of these enrichment approaches in assessing the qualitative and quantitative changes of glycoproteins in different types of cancers are presented and discussed. This chapter highlights the importance of glycoprotein enrichment techniques for the identification and characterization of new reliable cancer biomarkers.

References

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Citations

Nov 1, 2016·Analytical and Bioanalytical Chemistry·Shiyue ZhouYehia Mechref
Jul 12, 2018·Proteomics. Clinical Applications·Mayank SaraswatSuvi Renkonen
Jul 18, 2019·Expert Review of Proteomics·Danielle A Scott, Richard R Drake
Oct 20, 2018·Proteomics. Clinical Applications·Iwona BelczackaVera Jankowski
May 29, 2018·Analytical and Bioanalytical Chemistry·Petr KozlikMiloslav Sanda
Oct 21, 2016·Electrophoresis·Alireza BanazadehYehia Mechref
Nov 21, 2018·Analytical and Bioanalytical Chemistry·Zeyu SunLanjuan Li
Apr 21, 2021·Glycoconjugate Journal·Gerda C M VreekerRob A E M Tollenaar
May 23, 2021·Clinics in Laboratory Medicine·Calvin R K BlaschkeRichard R Drake
Jun 20, 2018·Journal of the American Society for Mass Spectrometry·Alireza BanazadehYehia Mechref
Dec 15, 2016·Chemical Reviews·Krishnan K Palaniappan, Carolyn R Bertozzi

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Methods Mentioned

BETA
glycosylation
bronchoalveolar lavage
ELISA

Software Mentioned

GlycoMapSera
GlycoFragWork

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