Defining ICR-Mo, an intrinsic colistin resistance determinant from Moraxella osloensis

PLoS Genetics
Wenhui WeiYoujun Feng

Abstract

Polymyxin is the last line of defense against severe infections caused by carbapenem-resistant gram-negative pathogens. The emergence of transferable MCR-1/2 polymyxin resistance greatly challenges the renewed interest in colistin (polymyxin E) for clinical treatments. Recent studies have suggested that Moraxella species are a putative reservoir for MCR-1/2 genetic determinants. Here, we report the functional definition of ICR-Mo from M. osloensis, a chromosomally encoded determinant of colistin resistance, in close relation to current MCR-1/2 family. ICR-Mo transmembrane protein was prepared and purified to homogeneity. Taken along with an in vitro enzymatic detection, MALDI-TOF mass spectrometry of bacterial lipid A pools determined that the ICR-Mo enzyme might exploit a possible "ping-pong" mechanism to accept the phosphoethanolamine (PEA) moiety from its donor phosphatidylethanolamine (PE) and then transfer it to the 1(or 4')-phosphate position of lipid A via an ICR-Mo-bound PEA adduct. Structural decoration of LPS-lipid A by ICR-Mo renders the recipient strain of E. coli resistant to polymyxin. Domain swapping assays indicate that the two domains of ICR-Mo cannot be functionally-exchanged with its counterparts in MCR-1/2 a...Continue Reading

References

Nov 1, 1986·The Journal of Antimicrobial Chemotherapy·R A Dixon, I Chopra
Jun 30, 2000·Journal of Molecular Biology·B StecE R Kantrowitz
Dec 24, 2003·Protein Science : a Publication of the Protein Society·Narasimha Sreerama, Robert W Woody
Jul 21, 2004·Journal of Computational Chemistry·Eric F PettersenThomas E Ferrin
Apr 13, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Matthew E Falagas, Sofia K Kasiakou
Aug 26, 2006·The Lancet Infectious Diseases·Jian LiDavid L Paterson
Sep 7, 2007·Cell·Michael A KohanskiJames J Collins
Mar 28, 2008·BMC Bioinformatics·Alexis Criscuolo, Olivier Gascuel
Mar 31, 2009·International Journal of Systematic and Evolutionary Microbiology·A I VelaJ F Fernández-Garayzábal
May 12, 2009·Nucleic Acids Research·Pascal BenkertTorsten Schwede
Dec 1, 2009·International Journal of Systematic and Evolutionary Microbiology·A I VelaJ F Fernández-Garayzábal
Jul 29, 2010·Proceedings of the National Academy of Sciences of the United States of America·Andrew M PowlB A Wallace
Sep 17, 2011·Journal of Chemical Information and Modeling·Roman A Laskowski, Mark B Swindells
May 17, 2012·Journal of Chemical Information and Modeling·John J IrwinRyan G Coleman
May 17, 2012·Proceedings of the National Academy of Sciences of the United States of America·Jessica V HankinsM Stephen Trent
Aug 22, 2012·Antimicrobial Agents and Chemotherapy·Timothy R SampsonDavid S Weiss
May 2, 2014·Nucleic Acids Research·Marco BiasiniTorsten Schwede
May 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Daniel J DwyerJames J Collins
Apr 29, 2015·Journal of Computational Chemistry·William J AllenRobert C Rizzo
Jun 24, 2015·Proceedings of the National Academy of Sciences of the United States of America·Michael A LobritzJames J Collins
Mar 24, 2016·Molecular Biology and Evolution·Sudhir KumarKoichiro Tamura
May 15, 2016·Science China. Life Sciences·Rongsui GaoYoujun Feng
May 28, 2016·Antimicrobial Agents and Chemotherapy·Patrick McGannKurt E Schaecher
Jun 28, 2016·The Journal of Antimicrobial Chemotherapy·Stefan Schwarz, Alan P Johnson
Aug 2, 2016·Lancet·Ramanan LaxminarayanJohn-Arne Røttingen
Aug 16, 2016·Science China. Life Sciences·Zhencui LiYoujun Feng
Aug 16, 2016·International Journal of Antimicrobial Agents·Sophie BaronAbiola Olumuyiwa Olaitan

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Citations

Feb 7, 2019·Frontiers in Microbiology·Yu-Zhang HeJian Sun
Feb 1, 2019·Communications Biology·Huimin ZhangYoujun Feng
Jan 12, 2020·Communications Biology·Huimin ZhangYoujun Feng
May 20, 2021·Cell Reports·Yongchang XuYoujun Feng
Jul 24, 2021·BMC Microbiology·Bruno G N AndradeRafael R C Cuadrat

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Datasets Mentioned

BETA
AIT43666

Methods Mentioned

BETA
circular dichroism
one hybrid
three hybrid
X-ray
PCR
affinity purification
4 hybrid
dissection

Software Mentioned

ICR
select
ChemDraw
MUSCLE
jModeltest
MEGA
UCSF Chimera
TMHMM
GRID
LigPlot +

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