Defining multidrug-resistant tuberculosis: correlating GenoType MTBDRplus assay results with minimum inhibitory concentrations

Diagnostic Microbiology and Infectious Disease
Priti KambliCamilla Rodrigues

Abstract

This study correlates MICs of rifampicin (RIF) and isoniazid (INH) with GenoType MTBDRplus assay results for drug-resistant Mycobacterium tuberculosis (MTB) clinical isolates. MICs of RIF and INH were established for 84 and 90 isolates, respectively, testing 7 concentrations of each drug. Genotypic resistance to each drug was determined by GenoType MTBDRplus assay with 50 representative mutations confirmed by pyrosequencing, with mutations in the rpoB gene associated with RIF resistance and mutations in the katG and/or inhA genes associated with INH resistance. Based upon the correlation of MICs with specific genetic profiles, relative resistance levels were established for each isolate. Results indicate that MTB phenotypic resistance, currently based upon the testing of isolate susceptibility to a single drug concentration, may be more accurately profiled via quantitative MICs, and therefore, the correlation of molecular diagnostic results with specific MICs may allow for more optimal treatment of infections.

References

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Citations

Jul 28, 2017·Tuberculosis and Respiratory Diseases·Kyung-Wook JoTae Sun Shim
Apr 8, 2017·Therapeutic Advances in Infectious Disease·Christopher GilpinK Weyer
Jan 21, 2021·PLoS Neglected Tropical Diseases·Qiao LiuJianming Wang
Jul 24, 2021·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Juliana Maira Watanabe PinhataRosângela Siqueira de Oliveira
Jul 13, 2020·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·E RivièreA Van Rie

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