Defining oligosaccharide specificity for initial sperm-zona pellucida adhesion in the mouse

Molecular Reproduction and Development
C D Thaler, Richard A Cardullo

Abstract

The identity of the sperm surface protein(s) responsible for sperm-zona pellucida binding in the mouse, as well as the characteristics of the oligosaccharide groups on zona pellucida glycoprotein 3 (ZP3) having ligand activity toward this receptor, remain controversial. Conflicting results from several groups have made interpretation of the current data difficult. By developing a quantitative binding assay to evaluate the molecular interactions between mammalian sperm and the zona pellucida during initial gamete interactions, we directly quantified sperm-ZP binding interactions at the molecular level for the first time. The ZP binding assay demonstrated that live, capacitated mouse sperm bind solubilized 125I-labeled ZP glycoproteins in a concentration-dependent manner characterized by a rapid forward rate constant of 3.0 x 10 (7)M-1 min-1. Following the initial characterization, the binding assay was used to examine the roles of the sperm surface enzymes galactosyltransferase (GalTase) and fucosyltransferase (FucTase) in sperm-zone pellucida binding in the mouse. These data indicate that substrates for FucTase, but not for GalTase, inhibit sperm-ZP binding, in contrast to earlier reports in which GalTase substrates significant...Continue Reading

References

Apr 1, 1990·Molecular Reproduction and Development·D A BenauB T Storey
Sep 1, 1989·Gamete Research·J M Baltz, R A Cardullo
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J D Bleil, P M Wassarman
Oct 1, 1985·The Journal of Cell Biology·L C LopezB D Shur
Oct 1, 1988·Biophysical Journal·J M BaltzR A Cone
Apr 1, 1986·The Journal of Cell Biology·J D Bleil, P M Wassarman
Nov 1, 1984·Developmental Biology·H M FlormanP M Wassarman
Apr 1, 1984·Biophysical Journal·D P Green, R D Purves
Nov 1, 1982·The Journal of Cell Biology·B D Shur, N G Hall
Nov 1, 1994·Biology of Reproduction·S S Raychoudhury, C F Millette
Oct 1, 1993·Molecular Reproduction and Development·S S Raychoudhury, C F Millette
Jul 11, 1993·Biochimica Et Biophysica Acta·M Jezequel-CuerG Durand
Mar 1, 1993·Developmental Biology·N TanphaichitrC A Lingwood
Sep 20, 1996·The Journal of Biological Chemistry·C D Thaler, R A Cardullo

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Citations

Sep 18, 1997·Molecular Reproduction and Development·F CattaneoM E Perotti
Mar 9, 2000·Journal of Reproductive Immunology·M R Barber, R A Fayrer-Hosken
Jul 24, 2008·The International Journal of Developmental Biology·Barry D Shur
Jun 25, 2002·Biology of Reproduction·Julierut TantibhedhyangkulNongnuj Tanphaichitr
Dec 26, 2001·Biology of Reproduction·Catherine D Thaler, Richard A Cardullo
Sep 18, 1998·Andrologia·E Töpfer-PetersenJ J Calvete

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