Defining species-specific immunodominant B cell epitopes for molecular serology of Chlamydia species

Clinical and Vaccine Immunology : CVI
K Shamsur RahmanB Kaltenboeck

Abstract

Urgently needed species-specific enzyme-linked immunosorbent assays (ELISAs) for the detection of antibodies against Chlamydia spp. have been elusive due to high cross-reactivity of chlamydial antigens. To identify Chlamydia species-specific B cell epitopes for such assays, we ranked the potential epitopes of immunodominant chlamydial proteins that are polymorphic among all Chlamydia species. High-scoring peptides were synthesized with N-terminal biotin, followed by a serine-glycine-serine-glycine spacer, immobilized onto streptavidin-coated microtiter plates, and tested with mono-specific mouse hyperimmune sera against each Chlamydia species in chemiluminescent ELISAs. For each of nine Chlamydia species, three to nine dominant polymorphic B cell epitope regions were identified on OmpA, CT618, PmpD, IncA, CT529, CT442, IncG, Omp2, TarP, and IncE proteins. Peptides corresponding to 16- to 40-amino-acid species-specific sequences of these epitopes reacted highly and with absolute specificity with homologous, but not heterologous, Chlamydia monospecies-specific sera. Host-independent reactivity of such epitopes was confirmed by testing of six C. pecorum-specific peptides from five proteins with C. pecorum-reactive sera from cattle...Continue Reading

References

Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S Henikoff, J G Henikoff
Jun 1, 1990·Journal of Clinical Microbiology·L A CampbellJ T Grayston
Sep 24, 1987·Journal of Immunological Methods·H M GeysenP G Schoofs
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W BaehrH D Caldwell
Oct 1, 1974·The Journal of Infectious Diseases·S P Wang, J T Grayston
Apr 7, 1999·Nature Genetics·S KalmanR S Stephens
Jul 21, 1999·European Journal of Obstetrics, Gynecology, and Reproductive Biology·J H WagenvoortM van de Cruijs
Nov 5, 1999·Clinical and Diagnostic Laboratory Immunology·A EssigR Marre
Feb 24, 2000·Nucleic Acids Research·T D ReadC M Fraser
Jan 11, 2003·Clinical and Diagnostic Laboratory Immunology·I PortigJ S H Gaston
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
Jan 29, 2005·Journal of Microbiological Methods·Dan LiBernhard Kaltenboeck
Nov 30, 2005·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Arthur A Andersen
Dec 7, 2005·Molecular and Cellular Probes·Ralf EhrichtKonrad Sachse
Feb 1, 2006·Proceedings of the National Academy of Sciences of the United States of America·Deborah D CraneHarlan D Caldwell
Feb 17, 2006·Methods : a Companion to Methods in Enzymology·Mohamed F Elshal, J Philip McCoy
Jun 13, 2006·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Yoshinao AzumaMutsunori Shirai

❮ Previous
Next ❯

Citations

Dec 1, 2015·Veterinary Microbiology·Rachid AazizKarine Laroucau
Aug 11, 2015·Veterinary Microbiology·A Rodolakis, K Laroucau
Jun 26, 2015·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·K LaroucauP Rolland
May 18, 2016·The Journal of Biological Chemistry·Kh Shamsur RahmanBernhard Kaltenboeck
Aug 3, 2018·MSphere·K Shamsur RahmanBernhard Kaltenboeck
Aug 17, 2021·The Journal of Infectious Diseases·Kh Shamsur Rahman, Bernhard Kaltenboeck

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.