Defining the effects of storage on platelet bioenergetics: The role of increased proton leak

Biochimica Et Biophysica Acta
Saranya RaviVictor Darley-Usmar

Abstract

The quality of platelets decreases over storage time, shortening their shelf life and potentially worsening transfusion outcomes. The changes in mitochondrial function associated with platelet storage are poorly defined and to address this we measured platelet bioenergetics in freshly isolated and stored platelets. We demonstrate that the hypotonic stress test stimulates both glycolysis and oxidative phosphorylation and the stored platelets showed a decreased recovery to this stress. We found no change in aggregability between the freshly isolated and stored platelets. Bioenergetic parameters were changed including increased proton leak and decreased basal respiration and this was reflected in a lower bioenergetic health index (BHI). Mitochondrial electron transport, measured in permeabilized platelets, showed only minor changes which are unlikely to have a significant impact on platelet function. There were no changes in basal glycolysis between the fresh and stored platelets, however, glycolytic rate was increased in stored platelets when mitochondrial ATP production was inhibited. The increase in proton leak was attenuated by the addition of albumin, suggesting that free fatty acids could play a role in increasing proton lea...Continue Reading

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Citations

Jun 20, 2018·The Biochemical Journal·Freyr JóhannssonÓttar Rolfsson
Oct 18, 2016·Current Opinion in Hematology·Richard O Francis
Oct 18, 2019·Cells·Jersey Heitor da Silva MauésRommel Mário Rodriguez Burbano
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Mar 20, 2021·Scientific Reports·Wouter A van der HeijdenQuirijn de Mast

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